Isolation of exosomal protein from cell culture media and body fluids is presently a tedious, non-specific, and difficult process. Empowered by leading technologies as well as years of experience in this field, Creative Biolabs has successfully launched an innovative exosomal protein isolation and profiling platform with various protein profiling technologies.

Why Need Exosomal Protein Isolation and Profiling?

Exosomes are extracellular membrane-bound nanovesicles originating from the intraluminal vesicles of multivesicular bodies secreted by diverse cell types under both normal and pathological conditions. Exosomes were shown to transport proteins, mRNAs, and microRNAs and to modulate immune reaction, angiogenesis, cell proliferation, and tumor cell invasion. Depending on the cellular origin, exosomes recruit various cellular proteins that can be different from the plasma membrane. Recently, an increasing number of exosomal proteins have been found to be potential biomarkers for a variety of diseases including cancer as well as liver and kidney diseases.

It is essential to investigate the release of exosomes from cell and characterize the exosomal markers. Consequently, their use in biomarker research as a source of disease-relevant cargo proteins shows great promise. The protocols for the isolation/purification and also for the lysis and subsequent solubilization of membrane proteins is required for protein biomarker discovery platform. The high yield of exosomal proteins could be used for further downstream applications including ELISA, protein mass spectrometry, protein biomarker verification, gel analysis of exosomal proteins, etc.

Fig.1 The mechanism of exosome formation and excretion.

High-end Technologies to Profile Exosomal Protein

Exosomes derive from monocytic leukemia cell line, umbilical vein endothelial cells, dendritic cells and embryonic stem cell-derived mesenchymal stroma cells, as well as serum, saliva, placenta and breast milk. Creative Biolabs offers professional technologies to isolate exosome and extract exosomal protein from various samples. Creative Biolabs also provides several exosomal protein profiling technologies outlined below. If you require, we provide high-end technologies mass spectrometry to help you identify exosome protein.

Features of Our Services

• Direct capture and exosome protein extraction from human biofluids and cell culture media
• Small volume of biological sample (plasma) per reaction
• High yield and quality protein
• Various protein profiling technologies

With the help of our well-established technologies and experienced scientists, Creative Biolabs is capable of developing exosomal protein isolation and profiling. With innovative exosome isolation method allowed to recover fully intact exosomes in higher yields, we accept biological sample including plasma, serum, urine and cell culture media. We provide very flexible options for each specific case. We are happy to make it accessible to all kinds of research and industrial customers. Please don’t hesitate to contact us for more information.

• RNA Isolation & qPCR Analysis
• cfDNA Isolation & Profiling
• Cytokines Profiling