Exosomes are selectively enriched in some transmembrane proteins that can be genetically engineered to display ligands/homing peptides on their surface, which confers exosome targeting capability to cells bearing cognate receptors. This is based on the exosome display technology to manipulate the molecular composition of the exosomes and tailor them with new desirable properties. To promote the research in targeted delivery of exosomes for gene delivery or drug delivery, Creative Biolabs offers high-quality development services of targeted exosomes based on exosome display technology.

Exosome Display Technology for Exosome Targeting

Exosome Display is a novel methodology enabling the manipulation of exosome protein content, which enables soluble, membrane-bound, trans-membrane or multimeric ligands or homing peptides efficiently expressed at exosome surface in a native conformation. One of the most attracting applications of exosome display technology is to produce exosomes bearing target ligands or homing peptides specific to recipient cells. The ligands or homing peptides can be inserted into the transmembrane proteins enriched on the surface of exosomes to form the fusion protein, subsequently, the fusion proteins are inserted into plasmids to transfect cells. The transfection cells will secrete the exosomes bearing the target ligands or homing peptides which have the targeting capability to cognate receptor cells. With the discovery of many peptides homing to diseased tissues or organs through phage display and in vivo or in vitro biopanning technologies, there is ample opportunity to explore the potential use of exosome for targeted gene therapy.

Fig.1 Flow chart depicting steps for exosome-mediated delivery of siRNA. (El-Andaloussi, 2012)

What Can We Do?

Creative Biolabs provides customized services for the generation of targeted exosomes based on the exosome display technology.

• Identification of Exosome targeting peptides

  It is key for the identification of exosome targeting peptides, which facilitates the effective and functional display on the exosome surface and further promote the process of exosome targeting.

• Construction of Recombination exosomes

  Based on exosome display technology, we can utilize the different exosomal transmembrane proteins (Lactadherin, LAMP-2b, and PDGFR) for fusion with a variety of interest ligand or homing peptide. And the fusion proteins can be effectively displayed on the surface of exosomes.

Features

• Custom-specific project designs that allow targeting to different tissues by using appropriate tissue-specific targeting moieties fused to different exosomal membrane proteins
• Expert scientists and staff that work closely with customers to provide satisfying service support
• High-quality and low-cost services
• The best after-sales service

Creative Biolabs has abundant experience in exosome-related research and has a comprehensive suite of research programs to aid in the brilliant studies of our clients. If you are interested in our services, contact us and start the conversation.

Reference:

1. El-Andaloussi.; et al. Exosome-mediated delivery of siRNA in vitro and in vivo. Nature protocols. 2012, 7(12):2112-2126.

• Exosome Display-based Approach for Antibody Generation and Isolation (ExoMAb)
• Exosome Display-based Protein Array Screen