Overview

Exosomes, 30-150 nm tiny vesicles, can be isolated and purified from plasma and other body fluids and are valuable biomarkers for therapeutic and diagnostic applications. With extensive experience in exosome preparation, Creative Biolabs has developed a size exclusion chromatography (SEC) platform to purify exosomes from plasma and other body fluids, reducing the co-purification of non-exosomal blood proteins and enhancing the purity and integrity of exosomes.

Size Exclusion Chromatography

Size Exclusion Chromatography (SEC), also known as molecular sieve chromatography, separates molecules in solution by size and sometimes molecular weight. It is commonly used for large molecules or macromolecular complexes such as proteins and industrial polymers. When an aqueous solution transports the sample through the column, the technique is called gel-filtration chromatography. If an organic solvent is used as the mobile phase, it is known as gel permeation chromatography.

Services

Exosomes Purification by SEC

In exosome purification, SEC is an effective tool for achieving high yield and high purification. SEC separates sample components based on their differential movement through a gel matrix. Smaller components penetrate the gel matrix more easily, resulting in longer retention and later elution times. Larger components, such as exosomes, cannot penetrate the gel matrix and are excluded, allowing for separation from smaller protein and nucleic acid contaminants. Combining SEC with ultrafiltration or ultracentrifugation can further enrich exosomes from various samples.

Fig.1 Schematic of size-exclusion chromatography-based exosome isolation.^1,2

Features

• State-of-art technology platform
• Experienced and professional technical team
• Best after-sale service
• High yield and low consumption
• Relatively integrated exosomes

High-purity and intact exosomes will contribute to your downstream analyses, including more accurate quantification of vesicles and proteomic characterization. Creative Biolabs' skilled experts can help global customers to isolate and purify exosomes from various body fluids based on the advanced SEC platform. If you have any demands in exosome preparation, please don't hesitate to contact us for more information.

FAQs

References

1. Yang, D.; et al. Progress, opportunity, and perspective on exosome isolation-efforts for efficient exosome-based theranostics. Theranostics. 2020, 10(8):3684-3707.
2. Distributed under Open Access license CC BY 4.0, without modification.

• Ultracentrifugation & Microfiltration
• Immunoaffinity Capture