Exosomes are 30-150 nm tiny vesicles that are available to isolate and purify from plasma and other body fluids and regarded as the useful biomarker for therapeutic and diagnostic application. With years of experiences in exosome preparation, Creative Biolabs has developed size exclusion chromatography (SEC) platform to purify exosomes from both plasma and other body fluids with decreased co-purification of non-exosomal blood proteins, thus increasing the purification and integrity of exosomes.

Size Exclusion Chromatography

SEC, also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. Usually, it is applied to large molecules or macromolecular complexes such as proteins and industrial polymers. Typically, when an aqueous solution is used to transport the sample through the column, the technique is known as gel-filtration chromatography, versus the name gel permeation chromatography, which is used when an organic solvent is used as a mobile phase. The chromatography column is packed with fine, porous beads which are composed of dextran polymers (Sephadex), agarose (Sepharose), or polyacrylamide (Sephacryl or BioGel P). The pore sizes of these beads are used to estimate the dimensions of macromolecules.

Exosomes Purification by SEC

In the process of exosome purification, SEC acts as a good tool that can extract exosomes with high yield and high purification. The principle of SEC is to separate sample components based on the differential rate of movement through a gel matrix of different sized components. The smaller a component, the more it is able to penetrate into the gel matrix, the longer it is retained in the column and the later the elution time. While the larger components including exosomes cannot penetrate into the gel matrix and are excluded. This means that exosomes may be separated from smaller protein and nucleic acid contaminants. Besides, the combination of SEC and ultrafiltration or ultracentrifugation can significantly enrich exosomes from various samples.

Fig.1 A cartoon illustrating the theory behind size exclusion chromatography.

Advantages

• State-of-art technology platform
• Experienced and professional technical team
• Best after-sale service
• High yield and low consumption
• Relatively integrated exosomes

High-purity and intact exosomes will contribute to your downstream analyses, including more accurate quantification of vesicles and proteomic characterization. Our skilled experts can help global customers to isolate and purify exosomes from various body fluids based on the advanced SEC platform. If you have any demands in exosome preparation, please don’t hesitate to contact us for more information.

• Exosome Purification via Ultracentrifugation and Microfiltration
• Exosome Purification via Immunoaffinity