Circular RNAs (circRNAs) Products
Circular RNAs (circRNAs) are a new type of endogenous RNAs, which have gradually become the focus of RNA research in recent years. Creative Biolabs is a world-leading company focused on providing the best circRNAs product solutions to customers in the global gene therapy field. Our circRNAs products have been successfully applied in many research projects. Our experienced scientists can also customize circRNAs products according to your specific needs.
Overview of CircRNAs
Increasing evidence reveals that circRNAs play crucial roles in cell differentiation, tissue homeostasis and disease development. Due to several remarkable advantages, such as higher nuclease stability and longer cellular half-life, circRNAs are attractive markers for disease diagnosis. In addition, the dysregulation of circRNAs is closely linked to various diseases, such as cancer, cardiovascular diseases and nervous system diseases, thus, circRNAs may become promising therapeutic targets. Currently, several strategies have been developed for studying circRNAs and targeting circRNAs for in vivo therapeutic purposes. Typically, circRNAs are usually overexpressed via plasmids and knocked down by RNA interference (RNAi). Direct synthesis and purification of circRNAs to overexpress circRNAs is also a commonly used strategy.
Fig.1 Strategies used to target circRNAs as a therapeutic approach in vivo.¹
Synthetic CircRNAs
Single-stranded linear RNA can be produced by enzymatic strategies or chemical synthesis, followed by RNA circularization using a number of different methods. Enzymatic strategies typically rely on in vitro transcription (IVT) reactions, which allow for the synthesis of longer RNAs at a lower cost. Chemical synthesis can directly introduce monophosphoric acid during the synthesis process, which facilitates cyclization. However, chemical synthesis can only produce short RNAs. In addition, several in vitro linear RNA precursor ligation methods have been developed, including chemical ligation, enzymatic ligation, and ribozyme methods. The resulting circRNA molecules can be delivered into target cells.
- Chemical ligation
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Enzymatic ligation
- T4 DNA ligase
- T4 RNA ligase 1
- T4 RNA ligase 2
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Ribozyme method
- Group intron self-splicing system
- Group II intron self-splicing system
- Hairpin ribozyme method
Features of CircRNAs Products
- Flexible synthesis scale and various synthesis lengths,
- Multiple in vitro linear RNA precursor ligation methods ensure high-quality circRNAs,
- Highly purified circRNAs products,
- CircRNAs can be designed and optimized for specific tissue expression,
- Custom base modification to reduce the immunogenicity of circRNA.
Applications
- CircRNAs as biomarkers of cancers
- CircRNA-based drug delivery systems
- CircRNAs as therapeutic targets
- circRNAs-based vaccines or drugs
Diversified circRNAs products are available in Creative Biolabs, including but not limited to reporter circRNA products. All of our products have high stability and translation efficiency, which can meet your various downstream applications. We have a team of experienced and well-equipped scientists dedicated to collaborating with customers around the world to meet your specific requirements. If you are interested in our circRNAs products, please contact us for more details.
Reference
- He, Alina T., et al. "Targeting circular RNAs as a therapeutic approach: current strategies and challenges." Signal transduction and targeted therapy 6.1 (2021): 185.
