Custom Nucleic Acid Nanostructure Service

Nucleic acid nanotechnology has been developed to be a promising strategy for RNA interference (RNAi). Creative Biolabs is fully competent and dedicated to serving as your one-stop-shop for the development of DNA/RNA nanostructure for RNAi, including rational DNA/RNA nanostructure design, synthesis, and characterization. We offer high-quality custom services by adjusting protocols to meet even the most specific requirements.

Characteristics of Nucleic Acid Nanostructures

• DNA nanostructures

Grown exponentially in the past decade, DNA nanotechnology has been developed to be a promising strategy to construct various nano-biomaterials with structural programmability, spatial addressability and excellent biocompatibility. The sequences are programmable and the interactions between DNA are predictable. All these intrinsic properties of DNA allow numerous DNA nanostructures to be self-assembled with precisely controlled sizes and shapes. Comparing to traditional therapeutics carriers, DNA nanostructures exhibit a few advantages as an emerging delivery system for RNAi. They typically own high capacity for payloads, excellent biocompatibility and biodegradability, as well as great stability under physiological conditions. In addition, the inherent compatibility with therapeutic short double-stranded RNA (dsRNA) is the most outstanding feature of DNA nanostructures. Therefore, DNA nanostructures can serve as effective scaffolds and nanoscale vehicles for siRNA delivery for efficient gene silencing.

• RNA nanostructures

RNA nanostructures can be programmed to exhibit defined sizes, shapes and stoichiometries from naturally occurring or de novo designed RNA motifs. These constructs can be used as scaffolds to attach functional moieties, such as ligand binding motifs and gene expression regulators. RNA has been used extensively to construct various nanostructures, some of which are functionalized toward therapeutic applications. The suitability of RNAs as a material for use in nanotechnology and nanomedicine is due to their chemical, structural and functional properties. Multivalent RNA nanoparticles can be a very promising vehicle for the delivery of multiple siRNAs to suppress multiple genes simultaneously. A publication has shown that several RNA and RNA/DNA nanocubes could be functioned with multiple dsRNAs and the siRNAs can be conditionally released. Furthermore, the RNA nanocubes can be tracked intracellularly using fluorophores. In addition, spherical nucleic acid nanoparticle conjugates have also shown promise for systemic siRNA delivery for treating diseases.

Service

Scientists from Creative Biolabs have developed a versatile strategy to prepare well-defined nucleic acid nanostructures. Effective solutions have been employed to functionalize nucleic acid nanostructures for therapeutic dsRNA delivery for RNAi. So far, several nucleic acid nanostructures of variable sizes and shapes have been synthesized and have shown functionality for siRNA delivery for efficient gene silencing.

Highlights

• Low cytotoxicity
• Effective to across the lipid bilayer of mammalian cells
• Effectively increase intra-tumoral apoptosis and reduce tumor burden and progression without adverse side effects
• Different DNA/RNA nanostructures of variable sizes and shapes can be synthesized

Nucleic acid nanostructures can provide an unparalleled platform to construct highly ordered and well-controlled systems for optimized delivery of therapeutic dsRNA. Creative Biolabs would like to share our knowledge with our clients in nucleic acid nanostructure design for RNAi to facilitate their meaningful researches. For more detailed information, please feel free to contact us or directly send us an inquiry.