Custom Aptamer Screening
Nucleic acid-based aptamers have emerged as a new class of targeting moieties due to their high specificity and avidity. So far, various aptamer guided RNA delivery systems have been utilized for RNA interference (RNAi). In terms of extensive experience in aptamer research, Creative Biolabs is proud to introduce our aptamer design service and aptamer short dsRNA conjugation service to all the customers who are interested in RNAi therapeutics.
Aptamer-mediated Small Interfering RNA (siRNA) Delivery System
Aptamer is a big class of RNA or DNA oligonucleotides that have a high selective affinity to the target proteins or receptors. As the nucleic acid versions of antibodies, aptamer possesses unique characteristics, such as easy synthesis, lack of immunogenicity, and small size, which makes it more adaptable for siRNA conjugation and delivery. Compared to antibody or proteins, siRNA conjugation with aptamer is easy to operate. siRNA can be attached to the aptamer by two approaches. One is direct conjugation to the end of the aptamer chain, and the other is its complementary assembly to append with the aptamer. The first aptamer siRNA conjugate was constructed using the latter strategy in which scientists directly in vitro transcribed prostate-specific membrane antigen (PSMA) aptamer and sense strand of siRNA, and then annealed with antisense strand of siRNA to form an aptamer siRNA conjugate-targeting prostate PSMA.
Figure 1. Schematic illustration of the intracellular siRNA delivery to a target cell and RNAi machinery using aptamer-siRNA chimeras and aptamer-conjugated, siRNA-loaded nanocarriers. (Sivakumar, 2019)
Custom Aptamer Design Service for RNAi at Creative Biolabs
Creative Biolabs has established several engineering strategies for aptamer design to increased RNA loading efficiency. Our scientists also have accumulated rich experience in constructing aptamer short dsRNA (such as siRNA, miRNA, ssRNA) conjugates to deliver them into tumor cells. When DNA aptamer is used as a targeting ligand, our scientists will optimize a chemical linker to link RNAi and aptamer together. Through direct conjugation to the end of aptamer chain or complementary assembly to append with the aptamer, we have accomplished several aptamer short dsRNA conjugates successfully, which have been turned to be efficient in vivo experiments.
Highlights
- Several engineering strategies for aptamer design
- Aptamer with increased RNA loading efficiency
- Aptamer RNA conjugate with high targetability
- Functional optimization of RNAi aptamer conjugate
- Fast turnover time
Aptamer-mediated RNA delivery system is a highly promising approach for targeted delivery for RNAi. With years of experience in aptamer design and conjugation, Creative Biolabs is fully competent and dedicated to serving as your one-stop-shop for the development of aptamer-mediated RNA delivery systems. For more detailed information, please feel free to contact us or directly send us an inquiry.
Reference
- Sivakumar, P.; et al. (2019). Targeted siRNA delivery using aptamer-siRNA chimeras and aptamer-conjugated nanoparticles. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 11(3):e1543.
