Circular RNA Analysis Services
After the circRNAs are noticed by the researchers, Creative Biolabs constructs an outstanding team with associated experience in RNA research of molecular biology and cellular biology immediately and investigates circRNAs at the frontline. Gradually, we establish specific platforms and reliable strategies from our existing techniques for circRNAs. We can provide the circRNAs synthesis service and related analysis services.
Circular RNA Analysis Services
Fluorescence in situ hybridization (FISH) is a technique that can be used to identify and localize particular RNA targets (circRNAs) in fixed and permeabilized cells. It can describe the spatiotemporal patterns of gene expression in cells in this setting. Fluorescent dyes or other kinds of conjugates coupled probes will be used to visualize a circRNA. The FISH will be highly specific when it is directed at the specific region of the circRNAs junction, while commonly detecting both circRNA and its linear compartment when targeting the identical region of the precursor.
For artificially synthesized circular RNAs, we can design and develop in vitro experiments to verify the function of circRNAs. It can be achieved in different existing platforms such as iPSC-derived cell models, organoids, primary cells, cancer cell lines, and immune cells to match different complex conditions of your project. Besides, we also provide functional verification of circRNAs in different directions like tumor killing, overexpression, neuroscience, cardiovascular disease, etc.
Through rationally designed experiments, common cell lines, such as HEK293, are used to verify the expression of artificially synthesized circRNAs in vitro, give the results, and provide rationalization suggestions based on our well-established cellular platform. For your customer circRNA, we can design appropriate positive and negative controls for better expression validation.
Fig.1 The expression of circRNA-EGFP in HEK293 cells 24 h after transfection.1
Why Choose Us?
- We can visualize the circRNA expression.
- Solve your critical questions about circRNAs and give out the most proper suggestions for your projects.
- Provide the custom study service, and do the expression validation to meet your demands.
- Timely provides experimental data that allows you to evaluate your project performance.
- High cost-effectiveness and short cycle time.
As a reputable biotech company, Creative Biolabs focuses on circRNAs, develops a knowledgeable team of trained professionals, and establishes clear methodologies in this area to meet all of your circRNAs analysis demands. Please feel free to contact us for more details about your project involving circRNAs analysis.
Reference
- Wesselhoeft, R. Alexander, Piotr S. Kowalski, and Daniel G. Anderson. "Engineering circular RNA for potent and stable translation in eukaryotic cells." Nature communications 9.1 (2018): 2629.
