Reporter Circular RNA Products
Circular RNA (circRNAs) has gradually become the latest research theme in the field of RNA in recent years. As a world-leading biotechnology company focusing on RNA biology, Creative Biolabs is committed to providing a variety of cost-effective circRNAs products, especially reporter circRNA products to help you make breakthroughs in RNA research.
Features of CircRNAs
Such RNAs are an extraordinary class of non-coding RNA molecules, featured by a closed loop structure. They are less susceptible to RNA exonucleases, allowing for more effective protein expression. In addition to natural circRNAs, synthetic circRNAs as well as some endogenous circRNAs can also encode proteins, which greatly improves the prospects of these molecules as gene expression platforms. Furthermore, they are more diverse than their linear mRNA analogs. Most circRNAs are highly conserved among distinct species, both at the sequence level and expression patterns. These traits suggest that such RNA molecules may act as crucial regulators at transcriptional levels, and may play a pivotal role in disease diagnosis.
Products Available at Creative Biolabs
Studies have revealed that circularization, translation efficiency, and protein stability can be improved by artificially engineering circRNAs. These special molecules can be used for a series of molecular tools or therapies. Creative Biolabs provides a variety of reporter circRNA products, including but not limited to:
- Luciferase reporter (LR) circRNA
LR genes are attractive tools for studying gene expression at the transcriptional level. They also play crucial roles in studying regulatory miRNAs complementary to specific circRNAs. The luciferase vector is constructed by inserting a specific circRNA sequence into a promoter-driven LR gene. This system provides a powerful tool for evaluating circRNAs as miRNA sponges.
Fig.1 The biogenesis of circRNA.¹
- Green fluorescent protein (GFP)-circRNA
Accumulating evidence shows that some circRNAs possess translation functions. The most popular method of generating a fluorescent reporter circRNA is to have it encode the sequence of a known fluorescent protein, such as GFP. CircRNA minigene reporter systems containing GFP play a pivotal role in confirming the translational potential of circRNAs. We can customize GFP-circRNAs according to your project needs, these products can efficiently express reporter proteins without causing severe cytotoxicity and immunogenicity.
CircRNAs have wide application prospects. As a leader in the field of RNA research, our reporter circRNA products provide a new direction for RNA research. With an advanced molecular biology experiment platform and experienced scientists, we promise to provide global customers with innovative circRNAs products with reliable quality and competitive prices. What's more, we also welcome specific requests, our professional team is ready to start your project. Please do not hesitate to contact us if you have any questions regarding our reporter circRNA products.
Reference
- Haque, Shahnaz, and Lorna W. Harries. "Circular RNAs (circRNAs) in health and disease." Genes 8.12 (2017): 353.
