Baculovirus Vector
Viral vectors have been utilized in gene therapy to delivery therapeutic genes to target cells through ex vivo or in vivo methods. The baculovirus is an insect virus that can efficiently transduce mammalian cells for transgene expression. It has emerged as a novel vector for gene delivery Because it is non-pathogenic to humans and causes minimal toxicity to mammalian cells. Creative Biolabs provides one-stop services of the scaled-up amplification, concentration, purification and formulation of baculoviruses to meet the needs of customers worldwide.
Baculoviruses are enveloped viruses with a circular dsDNA genome. As the model species of baculoviruses, the autographa californica multiple nucleopolyhedrovirus (AcMNPV) in a mammalian expression cassette is highly effective in mediating transient transgene expression in human cells. The baculovirus vector genome is approximately 130 kb with a large packaging capacity (allowing at least 38 kb of inserts), especially suited for short-term and high-level transgene expression.
Fig.1 Putative model of internalization of baculoviruses into mammalian cells
- Baculovirus Vector Applied in Vaccination
As vaccine carriers, baculovirus vectors can simultaneously stimulate the humoral and cellular immune responses for the treatment of influenza, malaria and rabies. Creative Biolabs provides numerous recombinant baculoviruses with different promoters and regulatory elements to enhance the expression of foreign proteins and boost the efficacy of vaccination.
- Baculovirus Vector Applied in Tissue Engineering
Baculoviruses can be transduced at extremely high efficiency in primary chondrocytes, bone marrow–derived mesenchymal stem cells (BMSCs), and adipose-derived stem cells (ASCs). By expressing various growth factors, baculovirus gene therapy can be used in tissue engineering and regenerative medicine, for example, to regulate cell differentiation and improve cartilage formation.
- Baculovirus Vector Applied in Cancer treatment
Baculoviruses also can express suicide genes, tumor suppressors or other antitumor genes at high efficiency in specific cancer cells. And we provide the design of inserts with immunepotentiating genes to elicit strong host immunity against malignancies of the bladder, brain, lungs and prostate.
Manufacturing Process of Baculovirus Vector
Creative Biolabs provides the cell lines Sf21, Sf9 and High Five to produce baculovirus vectors and recombinant proteins for both research and manufacturing uses. We have established high-efficient propagation methods and a range of product testing procedures to ensure the production of high titers of baculovirus vectors free of endogenous viruses and incidental contaminants.
Fig.2 Flow chart of the manufacturing process of baculovirus vector
Highlights
- Circumvents the vector neutralization
- High titers of baculovirus vector
- Large Insert capacity
- Low side effects
Creative Biolabs has been committed to developing multiple cutting-edge modification baculovirus vectors. We provide fast and accurate analytical methods for monitoring and optimizing the production process to maximize output while reducing costs. Please feel free to contact us and our scientists will work with you in research about gene therapy.
References
- Kwang, T. W.; et al. (2016). Manufacturing of AcMNPV baculovirus vectors to enable gene therapy trials. Molecular Therapy-Methods & Clinical Development, 3, 15050.
- Ono, C.; et al. (2018). Baculovirus as a Tool for Gene Delivery and Gene Therapy. Viruses, 10(9), 510.
