Scientists in Creative Biolabs have constructed a series of high quality yeast surface-displayed human antibody libraries for de novo discovery of therapeutically relevant antibodies. They’re available for license or contract service.
Table 1: Premade Human Antibody Libraries
|Catalog #||Type||Ab Format||Size||# Donor||Note|
|Custom HuscL-YDP-3®||naïve||scFv||∼1011||56 donors||Enriched in scFv of client’s interest|
|HuscL-YDP-4®||naïve||scFv||2× 106||60 donors||It's an anti-DR5 pseudo-immune human single-chain fragment variable (scFv) library on the yeast cell surface.|
|HuscL-YDP-7®||imumune||scFv||∼109||1 donor||Gene for this library is isolated from the bone marrow mononuclear cell of an HIV-infected donor who exhibits broad serum neutralization of HIV-1.|
|HuFabL-YDP-1®||naïve||Fab||over 109||35 donors||
The human LC and HC cDNAs were
harvested from a pool of 35 autoimmune patients.
|HuFabL-YDP-2®||synthetic||Fab||1.2 × 109||N/A||It's a yeast surface-displayed combinatorial Fab-1 with 11 CDR-H3 residues library.|
|HuFabL-YDP-3®||synthetic||Fab||1.1 × 109||N/A||It's a yeast surface-displayed combinatorial Fab-2 with 9CDR-H3 residues.|
|HuscFabL-YDP-1®||imumune||scFab||∼109||1 donor||Gene for this library is isolated from the bone marrow mononuclear cell of an HIV-infected donor who exhibits broad serum neutralization of HIV-1.|
|HuIgGL-YDP-1®||naïve||full-length IgG||1.5 × 109||N/A||This full-length IgG yeast surface-displayed library is constructed without requiring a genetically encoded anchor protein or intracellular antibody modification.|
|HuIgGL-YDP-2®||naïve||full-length IgG||∼109||150 donors||N/A|
|HuFn3L-YDP-1®||naïve||Fn3||6.5 × 107||N/A||Fn3 refers to the 10th type III domain of human fibronectin.|
These libraries have a size ranging from 106 to 1011 and cover most commonly used antibody formats, including scFv, Fab, scFab, full-length IgG and Fn3. Comprehensive library types and various genetic backgrounds give these libraries multiple functions and applications. In combination with the Ultraff™ Yeast Display Technology or Yeast Mating Technology from Creative Biolabs, low nanomolar, even picomolar-affinity antibodies of different formats are routinely obtained. All human antibody libraries for yeast display licensed from Creative Biolabs can be amplified 1010 fold under proper operation, without measurable loss of clonal diversity, allowing effectively indefinite expansion. Creative Biolabs’ top quality human antibody libraries for yeast display can improve your results and save your time in novel antibody discovery.
Figure 1. Four types of antibody libraries(Ref: High Affinity, Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation )
Advantages of Yeast Display:
For license or contract service, please contact us.