For label-free interaction measurements, Creative Biolabs provides surface plasmon resonance (SPR) service based on our unique ProteOn™ XPR36 protein interaction array system. Creative Biolabs has extensive experience in providing SPR services. You can count on us to assist you through your project.
SPR is an optical phenomenon which allows monitoring binding of any two unlabeled molecules in real time. The SPR signal is based on changes in the refractive index at the surface of a gold sensor chip as an analyte flows in a microfluidic channel and binds to a ligand immobilized on the sensor chip. Monitoring the changes in the SPR signal over time results in a plot of the binding response versus time called sensorgram. Fitting of the sensorgram data allows calculation of binding parameters, such as KD, Kon and Koff. On top of the kinetic constants, SPR measurements can be applied for:
Our ProteOn™ XPR36 system is a unique application of SPR that uses enhanced microfluidics and a 6 × 6 interaction array on the sensor chip to characterize the binding of multiple analytes to multiple ligands, in a single step, on a single chip. It means that Proteon allows to measure 36 interactions at the same time, which saves not only time, but also your sample.
Experienced staff at Creative Biolabs will help you through your project, including method development, validation, analysis of delivered samples and transfer to the client upon request. We can provide ready-to-publish or ready-to file data in any format required. Please see below for a list of services we provide.
Label-free interaction studies:
Service dedicated for comparability studies of biosimilars: