Close

Hybridoma Services

Creative Biolabs is one of the well-recognized experts who are professional in applying hybridoma technology for monoclonal antibody (mAb) development. Based on our extensive experience and advanced platform, we are proud to offer hybridoma services with different species for our customers all over the world.

Introduction of Hybridoma Technology

Hybridoma technology is a traditional method for the long-term production of large-scale mAb. Derived from the fusion of specific antibody-producing B lymphoblast with myeloma, the forming of the hybrid cell line is the key part of hybridoma technology. The general development of hybridoma cells starts with animal immunization and then the splenocytes would be harvested. The B cells that produce specific antibodies are isolated and in turn fused with myeloma. The obtained stable fusion cells are known as hybridoma cells, which can express antigen-specific mAb continuously. MAbs are of great value not only in basic research of biology and immunology but also in medical applications. In addition, equipped with other advanced techniques available in Creative Biolabs, such as antibody-drug conjugates (ADCs), hybridoma sequencing, and bioconjugation the extended application of mAbs can further assist the development of either therapeutic or diagnostic agents.

Hybridoma technology. (Saeed, et al., 2017)Fig.1 Hybridoma technology.1

One-stop MAb Production by Hybridoma

Our scientists have extensive experience in handling various types of antigen. We can work with the purified antigen or start with the provided in silico sequence. The soluble proteins and peptides can be generated through our advanced recombinant/synthesis system. In terms of the novel Magic™ membrane protein production services, Creative Biolabs can produce membrane protein with various formats to meet our clients' requirements. Comprehensive Immunogen Design Services are available for you.

To meet our clients' specific project demands, we can tailor comprehensive immunization strategies which including but not limited to DNA immunization, whole cell immunization, and other conventional immunization strategies.

Our optimized fusion protocols enable the fusion of splenocytes with proper myeloma cells. Various protocols can be performed for the positive hybridoma screening, including immunoblotting, immunoprecipitation, ELISA, and IHC-positive hybridoma screening.

The antibody preparation (in vivo or in vitro) and purification will be conducted based on customer needs. High-quality antibodies will be delivered.

Features of Hybridoma Services from Creative Biolabs


Creative Biolabs has been a long-term expert in the field of mAb generation. Equipped with our advanced hybridoma platform, we are confident in offering various hybridoma-based services. If you are interested in our services, please do not hesitate to inquire us for more details.

Reference

  1. Saeed, Abdullah FUH, et al. "Antibody engineering for pursuing a healthier future." Frontiers in microbiology (2017): 495.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

Online Inquiry
CONTACT US
USA:
Europe:
Germany:
Call us at:
USA:
UK:
Germany:
Fax:
Email:
Our customer service representatives are available 24 hours a day, 7 days a week. Contact Us
© 2024 Creative Biolabs. | Contact Us