Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsCreative Biolabs has established a solid platform for scFv and Fab construction. There are three routes to have a scFv or Fab targets a specific antigen. The first approach is to generate a mouse hybridoma clone, then convert full IgG (or IgM) into a scFv or Fab; the second approach is to create an immunized phage display scFv or Fab mouse library, then use the antigen to screen the library; the third method is to use the antigen to screen a premade scFv or Fab antibody phage display library (usually a human one) to get scFv or Fab clones directly. We are also able to convert chicken IgY into scFv or Fab fragments.
Fab fragments differ from scFv's as they contain both variable domains and constant regions, thus their construction and ultimate expression is somewhat more complex. Creative Biolabs is experts with many years of experience in antibody production. The same heavy and light variable chains used for scFv construction can be used in the construction of Fab.
If a monoclonal antibody is available, the Fab fragment can be created enzymatically. papain is a cysteine protease enzyme that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge reason. This cleavage results in the generation of three ~50kDa fragments, two Fab domains and a Fc domain. The papain-digested antibody is unable to promote agglutination, precipitation, opsonization, and lysis. Immobilized papain offers the advantage of generating Fab and Fc fragments without the need to remove the papain enzyme after digestion.
Expression strategies:
- Secreted expression in periplasm of E. coli.
- Soluble expression in cytoplasm of E. coli.
- Insoluble expression as inclusion bodies.
- SUMO Fusion Platform.
Purification strategies:
- Affinity tag: Hexahistidine (His6) tag.
- Molecular format: insertion of SUMO between His6 tag and protein of interest (POI).
- Purification: IMAC (Immobilized Metal Affinity Chromatography).
- Tag removal after purification: cleavage at the C terminus of SUMO with SUMO protease.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
