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Membrane Protein Solubilization and Stabilization Reagents

Detergent Phospholipid Nanodisc Polymer Applications

Membrane proteins mediate processes that are fundamental for the flourishing of biological cells, playing critical roles in cellular function and therapeutic targets. Membrane-embedded transporters move ions and larger solutes across membranes; receptors mediate communication between the cell and its environment and membrane-embedded enzymes catalyze chemical reactions. One outstanding challenge is the difficulty in preparing a sufficient amount of these membrane proteins so as to be able to generate the functional antibody and conduct functional assays.

Membrane Protein (Creative Biolabs Original)

Detergent Products

Detergent-solubilization is a routine procedure for membrane protein isolation in most cases. We provide unique solubilization

reagents to screen for the best outcome.

Cat Product Name CAS Number CMC in H2O
MPD0025K n-Octyl-β-D-Glucopyranoside (OG) 29836-26-8 0.53%
MPD0041K n-Dodecyl-β-D-Maltopyranoside (DDM) 69227-93-6 0.0087%
MPD0044K n-Decyl-β-D-Maltopyranoside (DM) 82494-09-5 0.087%
MPD0070K Lauryl Maltose Neopentyl Glycol (LMNG) 1257852-96-2 0.001%
MPD0092K CHAPS 75621-03-3 0.49%
MPD0182K GDN 1402423-29-3 0.0021%
MPD0204K Octaethylene Glycol Monododecyl Ether (C12E8) 3055-98-9 0.0048%

Phospholipid Products

Phospholipids are one of the most frequently used formats for stabilizing membrane proteins, which is well applicable to immunization, assays, and diverse usages.

Cat Product Name Classifications CAS Number
MPL0001K DPPC Phosphatidylcholine (PC) 63-89-8
MPL0002K DSPC Phosphatidylcholine (PC) 816-94-4
MPL0005K DOPC Phosphatidylcholine (PC) 4235-95-4
MPL0006K POPC Phosphatidylcholine (PC) 26853-31-6
MPL0007K DAPC Phosphatidylcholine (PC) 61596-53-0
MPL0009K DSPE Phosphadylethanolamine (PE) 1069-79-0
MPL0089K DSPG Phosphatidylglycerol (PG) 200880-42-8
MPL0092K DLPG Phosphatidylglycerol (PG) 322647-27-8
MPL0093K DSPA Phosphatidic Acid (PA) 108321-18-2
MPL0096K DLPA Phosphatidic Acid (PA) 108321-06-8
MPL0097K DOPA Phosphatidic Acid (PA) 108392-02-5
MPL0098K DPPS Phosphatidylserine (PS) 145849-32-7
MPL0099K DOTAP Cationic Lipids 132172-61-3

Nanodisc Products

Nanodiscs aim at stabilizing membrane proteins in mimic lipid bilayers with a more dense environment that is even closer to the natural membrane environment.

Cat Product Name Species Tag Nanodiscs Size
MPN0001K MSP1E3D1, His- Streptomyces kanamyceticus His tag at N-terminus 12.9 nm in diameter
MPN0002K MSP1D1, His- Microbial His tag at N-terminus 9.7 nm in diameter
MPN0016K MSP1D1 ∆H5, tag-free Human Tag-free 7-8 nm in diameter
MPN0017K MSP1D1, tag-free Mouse Tag-free 9-10 nm in diameter
MPN0024K MSP1E3D1, tag-free Human Tag-free 12-14 nm in diameter
MPN0025K MSP2N2, His Human His tag 17 nm in diameter

Polymer Products

Polymer technique can solubilize hydrophobic membrane proteins without detergent, meanwhile maximally preserving native confirmation and bioactivity.

Cat Product Name Molecular Formula Formula Weight
MPP010K Amphipol A8-35 C6.2H10.3O1.35N0.65Na0.35)~72 9 kDa
MPP011K Poly (Maleic Anhydride-alt-1-Decene) substituted with 3-(Dimethylamino) Propylamine (C19H36O3N2)n 18,500.00
MPP012K Poly (Maleic Anhydride-alt-1-Tetradecene) substituted with 3-(Dimethylamino) Propylamine (C23H44O3N2)n 12,000.00
MPP013K Poly (Maleic Anhydride-Alt-1-Octadecene) substituted with 3-(Dimethylamino) Propylamine (C27H52O3N2)n 39,000-65,000
MPP014K Biotinylated Amphipol A8-35 C6.5H11.225O1.375N0.75Na0.325)~72 9.3 kDa
MPP015K Poly (Maleic Anhydride-alt-1-Decene) substituted with 3-(Dimethylamino) Propylamine and Biotin ethylene diamine (C19H36O3N2)n.(C26H43O5N4S)m 18.5 kDa
MPP016K Non-Ionic Amphipol A8-35 C7H15O4SN (C31H54O15N2)n 7000-35000g/mol

Applications of Different Membrane Protein Solubilization Forms

Detergent Form Liposome Form Nanodisc Form Polymer Form
  • X-ray Protein crystallization
  • Solution-state NMR
  • Enzyme kinetics
  • Protein engineering and
  • mutagenesis studies
  • Drug screening
  • Drug target discovery
  • Diagnostic application
  • Electron microscopy
  • Solid-state NMR
  • Transport assays
  • Therapeutic application
  • Drug screening
  • Protein-protein interaction experiment
  • Small molecule or drug target discovery
  • Immunization & antibody selection
  • Studies of protein-protein/protein-lipid/protein-ligand/protein-additive interactions
  • Membrane protein delivery
  • Studies of membrane protein conformation dynamics
  • Discovery of small-molecule drugs
  • Protein structure with Cryo-EM
  • Protein-lipid interaction

All listed customized services & products are for research use only, not intended for pharmaceutical, diagnostic, therapeutic or any in vivo human use.

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