As a well-recognized expert at phage display technology, Creative Biolabs offers our worldwide clients the best phage display library screening services through our excellent biopanning strategies and state-of-the-art equipment. Our scientists are pleased to tailor the most appropriate strategies to screen the interested libraries of our customers.
Acceptable Library Types
According to different demands, a series of targets can be qualified screened by our service. We accept targets provided by clients, but also provide related services to synthesize and express the interested targets.
Acceptable Target Types
Phage display technology is one of the major approaches employed in various protein interaction studies, especially in the discovery of specific antibodies, scaffolds or ligands. While binders with the desired specificity generally exist at low frequencies in the constructed libraries. In this way, phage display library screening has become an effective technique for the enrichment and identification of binders with high affinity and specificity from interested libraries.
At Creative Biolabs, the proprietary protocol and tailored biopanning strategy allow the increase of candidates with higher affinity and desired specificity (e.g. binders recognize conformational epitope rather than linear epitope). In general, by conducting four rounds library screening, our scientists can select scFv/Fab antibodies with the affinity of 10-7. Through constructing serial sub-libraries of the isolated scFv/Fab antibodies, we can increase the affinity from 10-8 to 10-9. Moreover, Creative Biolabs has successfully obtained a scFv antibody with an extremely high affinity of 10-12.
Based on our standard biopanning protocol, Creative Biolabs can utilize different strategies for library screening to meet our clients’ project goals.
Solid-Phase Screening : the most straightforward and widely used way to select binders against interested target adsorbed onto a solid surface. Typically, the targets can be coated on the well of immunotubes or microtiter plates for isolating specific binders.
Solution-Sorting Screening: an ideal approach to isolate binders recognize naïve targets. The target-binder interaction is carried out in solution with subsequent capture by the appropriate method.
Cell-Based Screening: a method in particular for cell surface antigen selection. It is a reliable approach to screen cell surface antigens with native conformation, especially when the antigen is hardly expressed as recombinant protein.
In vivo Screening: a high throughput method for efficiently isolating tissue-specific binders. The selection is based on binding to target in vivo after the injection of interested phage libraries into whole animals, and then rescuing phage bound to specific organs and tissues.
Ex vivo Screening: a novel technique to employ primary cell suspensions of organs and tissues as targets. All cell types can be exposed for selection to identify the most specific binders.
Featured Screening Services at Creative Biolabs
Other Applications of Our Library Screening Service
In terms of years of experience in phage display realm, Creative Biolabs utilizes its advanced technology platforms and specialized expertise to serve our global clients with high-quality library screening services. Our scientists are confident in tailoring our customers the most reliable and cost-effective protocol to facilitate their meaningful research.