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Membrane Protein Solubilization and Stabilization

Unlike water-soluble proteins, most membrane proteins cannot be solubilized or stabilized in aqueous solutions without the aid of any amphipathic materials. One of the most common difficulties in handling membrane proteins is the choice of amphipathic materials. Amphipathic materials can play an indispensable role in the extraction, solubilization, stabilization, characterization, functionalization, and activation of membrane proteins.

membrane protein form (Creative Biolabs)

Membrane Protein Reconstituted in Detergent

Detergent-solubilization is a routine procedure for membrane protein isolation in most cases. Selection of appropriate detergent is very crucial as misuse of detergent might compromise protein structure, resulting in denatured products. With our unique reagents as well as other commercially available detergents, we can perform up to 20 solubilizing conditions to screen for the best outcome.

Membrane Protein Reconstituted in Detergent (Creative Biolabs)

Membrane Protein Reconstituted in Liposomes

Proteoliposomes are one of the most frequently used formats for stabilizing membrane proteins, which is well applicable to immunization, assays, and diverse usages. We have several different lipid compositions available (natural or artificial) for membrane protein reconstitution. Aided by our well-established protocol and vast experiment, the protein products can be incorporated into lipids with correct folding and high bio-activity.

Membrane Protein Reconstituted in Liposomes (Creative Biolabs)

Membrane Protein Reconstituted in Nanodiscs

Nanodiscs are a bunch of membrane protein-lipid complex that are held together by a belt-like membrane scaffold protein (MSP). Compared to detergent and liposomes, nanodiscs format can offer a more compact, native-like environment. Therefore, they are highly stable, controllable, and monodisperse, which is very suitable for many kinetics and functional studies.

Membrane Protein Reconstituted in Nanodiscs (Creative Biolabs)

Membrane Protein Reconstituted in Polymer

Amphipols are polymers produced by chemically linking octylamine and/or isopropylamine to a portion of the carboxylic groups of low-molecular-weight polyacrylic acids via the formation of amide bonds. They can solubilize membrane proteins by wrapping them on their hydrophobic surface in aqueous solutions.

Membrane Protein Expression in VLPs

Membrane Protein VLPs (MP-VLPs) are non-infectious virus-like particles (VLPs, about 150 nm in diameter) that display highly-expressed copies of specific membrane proteins in their native conformation. MP-VLPs are produced from mammalian cells by co-expressing the retroviral structural core polyprotein (gag) and the target membrane protein. Retroviral gag core proteins self-assemble at the plasma membrane, where they bud from the host cells over-expressing a membrane protein of interest, enabling the formation of MP-VLPs.

Membrane Protein Reconstituted in Polymer (Creative Biolabs)

Screening for the best detergent or micelle-forming lipid to construct micelles solubilizing a membrane protein can be quite a labor- and capital-intensive task. Each detergent or micelle-forming lipid may have very different stabilizing or destabilizing effects on a membrane protein. Besides, in most cases, different micelles need to be used for membrane protein extraction from different sources, as well as for different applications. With rich experience in this area and a series of detergents and lipids available, Creative Biolabs has built comprehensive detergent screening platform and lipid screening platform to find the best micelles for our customers' specific membrane protein targets with high purity, stability, and yield. Please feel free to inquire us for more information.


All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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