Creative Biolabs has extensive experience to offer the novel human or humanized antibody services for either therapeutic or laboratory applications. We have three routes in developing human antibodies. The first and most straightforward method is to screen our human antibody libraries. The second method is to immunize premade “humanized” mice and then raise hybridoma cell lines [or to create an immune humanized antibody library] that produce human antibodies. The third method is antibody humanization. We rely on animals to produce non-human antibodies using either hybridoma or immune antibody library screening and screening methods; after that, the top candidate is humanized.
Through the flourish of antibody based therapeutic drugs, the demand of human or humanized antibody is synchronized growth. Due to the potential risk of immunogenicity, the full human antibody or at least humanized antibody (from xenogeneic sources) is required for further clinical application. Because of the serious ethical and moral issues, it is obviously an impossible task to directly immunize human beings with interested antigens. Nevertheless, several techniques have been developed as alternative solutions to overcome this limitation. Creative Biolabs is able to provide human or humanized antibodies in the ethical standards category, three main aspects have been enumerated as followed:
Truly Human Antibody from Premade Human Antibody Libraries
Creative Biolabs owns several naive or synthetic human antibody libraries in-house with diversity of over 109. These premade libraries are generally based on human antibody genes from non-immunized donors or human antibody repertoire and constructed in scFv or Fab format. In this way, these premade libraries are qualified for screening human antibodies specific binding to the target protein (or other molecules). Owe to the 100% human sources, the isolated antibodies carry rare risk to cause significant immunogenicity issue in therapeutic applications.
Fully Human Antibody from FHAT Transgenic Mice
Another choice for producing fully human antibody is utilizing the Fully Human Antibody Technology™ (FHAT) of Creative Biolabs. This technology is based on the creation of a mouse strain engineered to produce a large repertoire of human antibodies in the absence of mouse antibodies. Through introducing human immunoglobulin loci into the germline of mice deficient in mouse antibody production, the “humanized” transgenic mice (FHAT transgenic mice) are able to produce significant levels of antigen-specific human antibodies with high affinity and specificity.
Non-human Primate (NHP) Antibody Humanization
Creative Biolabs has developed the innovative Hi-AffiTM platform to provide top-class humanization of non-human primate (NHP) antibody as a unique solution to develop therapeutic antibodies for human use. Compared with the commonly used rodent, NHP has much closer genetic relationship to that of human, which means that the antibodies isolated from immunized NHPs may have higher affinity and lower immunogenicity to human beings. On the other hand, the humanization of NHP antibody is much easier than other species, and potentially has no affinity loss under suitable technique.
Besides non-human primate, Creative Biolabs also provide humanization service to antibodies derived from other species, such as mouse, rat, rabbit, chicken, llama, dog, shark and more.