With years of experience in the field of antibody development, Creative Biolabs has established a high-accuracy sequencing platform to provide top quality antibody sequence determination services from hybridoma cell lines. Creative Biolabs’ hybridoma sequencing service is highly versatile and is applicable for both IgG and IgM types of monoclonal antibodies produced by mouse and rat hybridoma cell lines. We are dedicated to providing first-class customized service to contribute to the success of your project.
The Value of Antibody Sequence
The protein sequence of an antibody is one of its most important attributes that enables various downstream applications and developments:
Hybridoma sequencing refers to the process of obtaining sequence information regarding the cDNA encoding the variable heavy (VH) and variable light (VL) domains of the antibody produced by your hybridoma cell line. Before sequencing, total mRNA of the hybridoma cells is extracted followed by PCR amplification of antibody variable regions (VH and VL) as well as non-variable flanking constant region sequences. The antibody sequence information is derived from the amplified PCR products by a sequencer. With antibody variable region protein sequence, different antibody formats can be designed by grafting the variable region onto various framework sequence templates. Antibodies generated by this method can be produced via recombinant expression in a mammalian expression system. Alternatively, the full-length sequence can also be derived from hybridoma sequencing to enable antibody production via recombinant expression and to serve as an insurance policy to secure the source of the antibody in case of losing the hybridoma cell line due to contamination or other reasons.
Fig 1. Schematic representation of the approach for high-throughput sequencing of the Ig sequence repertoire. (Georgiou, G., 2014)
Hybridoma sequencing service at Creative Biolabs:
In this service, the 5'RACE method is applied to amplify, clone and sequence the complete sequences of both heavy and light chains of an antibody, which are subsequently cloned into two cloning vectors for downstream sequence manipulation and expression vector constructions.
In this service, only the sequences of the heavy and light chains at the variable region of an antibody are amplified by specially designed degenerated primers and sequenced. The resulted variable region can be grafted into any framework to yield an Ig isoform that is most suitable for the client’s project.
With the antibody sequence, scientists at Creative Biolabs provide services to convert the sequences into various forms of antibodies, including multi-isoform full-length antibodies and fragment antibodies such as Fab or scFv. A detailed project report, as well as vectors containing the antibody constructs, are delivered to the client at the end of the project.
Creative Biolabs also offers high-throughput hybridoma platform and other hybridoma-related services to facilitate your antibody discovery projects. Please feel free to contact us for more information.