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Custom Antibody & Hybridoma Services

Hybridoma technology is a technology of forming hybrid cell lines (hybridomas) by fusing a specific antibody-producing B cell with a myeloma (B cell cancer) cell that is selected for its ability to grow in tissue culture and for an absence of antibody chain synthesis. The antibodies produced by the hybridoma are all of a single specificity and are therefore monoclonal antibodies.

Creative Biolabs provides a full range of services in custom polyclonal and monoclonal antibody production from gene expression or peptide synthesis to antibody purification and labeling. We can produce antibodies in either research quantities or on a large scale for the diagnostic industry. We do epitope prediction, peptide synthesis, protein expression, polyclonal or monoclonal antibody production as well as antibody purification from cell culture, ascites fluid, or whole serum.

Workflows of Hybridoma Technology

Hybridoma technology produces monoclonal antibodies against antigens. The monoclonal antibodies prepared by this method are highly specific and derived from the monoclonal antibodies of the parental B cells.

Fig.1 Flow chart of hybridoma technology. (Creative Biolabs Original)Fig.1 Flow chart of hybridoma technology.

Antigen Preparation
  • Peptides, proteins, enzymes, compounds, DNA, antibodies and eukaryotic cells
  • Immunogen develpment: Peptides analysis & synthesis, customized recombinant protein expression, Haptens-KLH/OVA conjugation, eukaryotic cell cluture
Animal Immunization
  • Mouse C57BL/6 and Balb/C
  • Rat Sprague-Dawley and Wistar
  • Immunodeficient mouse
  • Humanization mouse
Hybridoma Screening
At least 2 rounds of specific ELISA screening and 2 rounds hybridoma subclone.
Function Guaranteed Hybridoma
  • Function-guaranteed hybridoma could be offered to client as an optional service after ELISA screening.
  • FACS, ICC, IHC, WB, flow cytometry assays are available for Hybridoma clones identification.
Additional options: Hybridoma Sequencing
Sequencing (covering variable regions: VH and VL) and report (both DNA and amino acid sequences, with CDR regions highlighted).
Delivery
  • At least 1-3 hybridoma cells
  • 1-5mg antibody from one clone choosen by the client
  • Up to 20 hybridoma supernatants (500ul-1ml) for antibody function verification
  • Complete project report
Lead time
90-120 days. The time may vary depending on the different requirment, and additional time should be allowed for additional validation testing or repetition, if necessary.

Case Study

Fig.2 2nd titration result-animal immunization. (Creative Biolabs Original)Fig. 2 2nd titration result-animal immunization.

Fig.3 ELISA screening of 2nd subclone. (Creative Biolabs Original)Fig. 3 ELISA screening of 2nd subclone.

The client provided a protein target X, we designed a peptide immunogen and did the function test of selected positive hybridoma. Fig.2 is the ELISA titration result in the immunization process. Fig.3 is the ELISA result of the finally selected hybridomas.

Fig.4 Reducing 12% SDS-PAGE analysis of purified recombinant protein. (Creative Biolabs Original)Fig. 4 Reducing 12% SDS-PAGE analysis of purified recombinant protein.

Fig. 5 Antibody Pair Screening. (Creative Biolabs Original)Fig. 5 Antibody Pair Screening.

The client provided the sequence of the target protein. Creative Biolabs was contracted to develop monoclonal antibodies targeting different epitopes on protein through mouse immunization and hybridoma screening.

Custom Antibody & Hybridoma Services Content

Liposome Immunization

As a highly optimized service, our liposome immunization technology relies on a proprietary liposome immunization procedure using liposome-encapsulated antigens, which including but not limited to the polysaccharide, lipid, glycolipid, and DNA.

Murine Hybridoma

Our proprietary mouse immunization approach allows us to provide mouse monoclonal antibodies within 70 days.

Rat Hybridoma

We have developed a proprietary hybridoma-generation platform for efficient development of high-affinity and high-specificity rat monoclonal antibodies.

Hamster Hybridoma

We have extensive experience in producing monoclonal antibodies in hamsters.

Guinea Pig Hybridoma

Through fusing with our specific myeloma as partner, guinea pig hybridoma can be developed with excellent stability and productivity.

Hybridoma Generation in Gene Inactivated Mice

The immunization of "knock-out" mice was an effective way to overcome the problem of inter-species sequence similarity in antibody production.

Anti-Small Molecules Hybridoma Development

We provide custom anti-small molecule mAb development services through hybridoma technology.

Function-guaranteed Antibody Development

It is important to determine the application and hybridoma screening method since not all antibodies will work with every application.

Hybridoma Rescue Service

We now have the capability and technology platforms to offer you diverse hybridoma rescue services.

Precision Antibody Replication Service

Immunogen design service is one of our core technical services. We offer complementary protein expression or peptide design services to generate custom antibodies with better performance.

Bulk Antibody Production Services

Delivering the final products with fast turnaround time and high quality.

Hybridoma Isotype Identification and Switching Service

We provide world-class packaging services for hybridoma isotype Identification and switching.

As an industrial leader specializing in custom services, Creative Biolabs also provides gene-engineered antibodies including single domain antibodies and humanized antibodies. Moreover, we can conduct phage display antibody library screening and antibody development by single B cell sorting platform. Contact us for more information.


All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.


All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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