Based on the extensive experience and advanced technical platforms, Creative Biolabs offers customized, one-stop ECIA™ cellular analysis services designed to accelerate your immune monitoring or epitope discovery projects. We carry out the cellular assays with optimized and validated protocols, which are time-saving and cost-efficient. Our experienced scientists could deliver accurate reports and overcome the pitfalls of variability. With our unique high-throughput approach, hundreds of samples can be processed in a single program, allowing you to focus on your critical work and project planning.
Upon receiving your inquiry, our experts will engage themselves with your project quickly, design suitable cell-based analysis protocols, come up with a complete quotation including experimental design, cell preparation and shipping, details of the assay protocols, and the final report. Customers can order a single cellular analysis service or any other service from the whole epitope discovery system.
Fig.1 Cell-mediated immunity. (Wen, 2017)
HLA tissue typing service is consisted of a series of systematically integrated assays to help customers acquire complicated HLA allele information for both academic research and clinical application.
We provide up-to-date HLA sequencing services and use the PCR-SSO/SSP strategies, which could meet specific requirements in sequencing resolution, time, budget, and research objectives and perfectly maintain a balance among the assay quality, time and cost. Furthermore, modules based on the Next-Generation Sequencing (NGS) are ready for a more precise analysis.
This assay can identify and enumerate the number of ASC and those secreting antibodies to a specific antigen in samples with high sensitivity at the cellular level. On the other hand, this platform can also be utilized for infection detection, drug therapy, vaccination, and autoimmunity.
The optimized ELISpot assay measures the magnitude and quality of T cells immunity through detecting antigen-specificity T cells that involve in the secretion of cytokines and other utility. This process is much more reproducible and sensitive than other assays, such as cytokine measurements in detecting low-frequency antigen-specific CD4+ and CD8+ T cells.
Intracellular cytokine staining is achieved by blocking the transfer of protein-mediated by the Golgi apparatus, by the use of in vitro polyclonal activators or specific antigen-stimulated cells and cytokine transmembrane secretion blocker, allowing the accumulation of cytokines in the cytoplasm.
This protocol can display the character of single-cell and the specificity of cell groups, which could directly reflect in vivo cell state and the level of protein in the cytoplasm.
Carboxyfluorescein succinimidyl ester (CFSE) can easily penetrate the cell membrane and covalently bind to the intracellular protein in the living cells. It will release green fluorescence after hydrolysis. The labeled cells can be observed for weeks in vivo so that they are normally utilized in the experiments of cell test and cell activity observation using fluorescent electron microscopy or confocal.
In animal research, the interaction between host and pathogen has attracted more and more attention. There has been some progress in the study of T-cell immune reagents for many animals, but it is urgent to directly evaluate pathogen-specific T-cells, a rare cell population, but the most important thing is to coordinate the host's immune response to specific pathogens. At present, we provide a technology that can quantify antigen-specific cellular immunity at the single-cell level, and can monitor individual immunity in detail based on the variability related to the number of activated T cells or the type of cytokines secreted. In veterinary medicine, the detection of pathogen-specific T cells can provide diagnostic help in the case of unclear or missing serological results.