Based on the extensive experience and advanced technical platforms, Creative Biolabs offers customized, one-stop ECIA™ cellular analysis services designed to accelerate your immune monitoring or epitope discovery projects. We carry out the cellular assays with optimized and validated protocols, which are time-saving and cost-efficient. Our experienced scientists could deliver accurate reports and overcome the pitfalls of variability. With our unique high-throughput approach, hundreds of samples can be processed in a single program, allowing you to focus on your critical work and project planning.
Upon receiving your inquiry, our experts will engage themselves with your project quickly, design suitable cell-based analysis protocols, come up with a complete quotation including experimental design, cell preparation and shipping, details of the assay protocols, and the final report. Customers can order single cellular analysis service or any other service from the whole epitope discovery system.
ECIA™ Cellular Analysis Service System
HLA Tissue Typing Service
HLA tissue typing service is consisted of series of systematically integrated assays to help customers acquire complicated HLA allele information for both academic research and clinical application.
We provide up-to-date HLA sequencing services and use the PCR-SSO/SSP strategies, which could meet specific requirements in sequencing resolution, time, budget, and research objectives and perfectly maintain a balance among the assay quality, time and cost. Furthermore, modules based on the Next-Generation Sequencing (NGS) are ready for a more precise analysis. Read more…
B Cell ELISpot Assay
This assay can identify and enumerate the number of ASC and those secreting antibodies to a specific antigen in samples with high sensitivity at the cellular level. On the other hand, this platform can also be utilized to infection detection, drug therapy, vaccination, and autoimmunity. Read more…
T Cells ELISpot Assay
The optimized ELISpot assay measures the magnitude and quality of T cells immunity through detecting antigen-specificity T cells that involve in the secretion of cytokines and other utility. This process is much more reproducible and sensitive than other assays, such as cytokine measurements in detecting low-frequency antigen-specific CD4+ and CD8+ T cells. Read more…
Intracellular Cytokine Staining Assay
Intracellular cytokine staining is achieved by blocking the transfer of protein mediated by Golgi apparatus, by the use of in vitro polyclonal activators or specific antigen-stimulated cells and cytokine transmembrane secretion blocker, allowing the accumulation of cytokines in the cytoplasm.
This protocol can display the character of single cell and the specificity of cell groups, which could directly reflect in vivo cell state and the level of protein in the cytoplasm. Read more…
CFSE Cell Proliferation Assay
CFSE can easily penetrate the cell membrane and covalently bind to the intracellular protein in the living cells. It will release green fluorescence after hydrolysis. The labeled cells can be observed for weeks in vivo so that they are normally utilized in the experiments of cells test and cells activity observation using fluorescent electron microscopy or confocal. Read more…
Flow Cytometry Testing Assay
Flow cytometry testing (FCT) is a technique for detecting single cell or other biological particles in suspension, which could be used for counting and sorting the tiny particles suspended in the fluid, or perform a variety of parametric analysis on a single cell flowing through an optical or electronic detector. By detecting labeled fluorescent signals, high-speed, one-by-one cell quantification and sorting can be achieved.
Fig.1 The Illustration of Flow Cytometry.