How does the cleavable linker benefit the functionality of the anti-Rat IgG(Fc)Fab-C-DMDM ADC?

The cleavable linker in the ADC allows for the controlled release of DMDM upon internalization into target cells. This feature ensures that the cytotoxic agent is activated only within the intended cells, enhancing the specificity of the ADC and reducing potential side effects on non-target cells.

What are the primary research applications of the anti-Rat IgG(Fc)Fab-C-DMDM ADC?

This ADC is primarily used for pre-screening rat monoclonal antibodies as potential ADC candidates in research settings. It enables efficient and economical testing of antibodies for their binding efficacy and therapeutic potential against cells expressing rat IgG Fc.

What role does glycosylation play in the functionality of the anti-Rat IgG(Fc)Fab-C-DMDM ADC?

Glycosylation at the Fc fragment of the antibody enhances its stability and interaction with Fc receptors on immune cells, which is crucial for mediating immune responses such as ADCC and opsonization. These interactions are vital for the effective functioning of the ADC in biological systems.

What is the significance of the anti-Rat IgG(Fc)Fab-C-DMDM ADC in the development of rat-specific immunotherapies?

This ADC plays a crucial role in the development of rat-specific immunotherapies by allowing researchers to screen and validate rat monoclonal antibodies that could be used in targeted therapies. Its ability to specifically bind and deliver cytotoxic agents to cells expressing rat IgG Fc receptors makes it an invaluable tool in designing therapies that are optimized for efficacy and specificity in rat models of disease.

What experimental setups are most suitable for testing the efficacy of the anti-Rat IgG(Fc)Fab-C-DMDM ADC?

The ADC's efficacy is best evaluated in cell-based assays that express rat IgG Fc receptors. These setups can include in vitro cytotoxicity assays, binding affinity tests, and potentially in vivo models designed to assess targeted drug delivery and therapeutic outcomes.

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anti-Rat IgG(Fc)Fab-C-DMDM ADC

anti-Rat IgG(Fc)Fab-C-DMDM ADC (CAT#: ADC-AA-044)

This ADC product is comprised of a Fab fragment of an anti-rat IgG Fc specific polyclonal antibody conjugated via a cleavable linker to DMDM. The antibody portion is a Fab fragment of a secondary antibody and the drug portion, DMDM, is a cytotoxic small molecule which binds to DNA, causes DNA damage. This product displays no obvious toxicity without a primary antibody and can be a quite efficient and economical alternative to pre-screening rat monoclonal antibodies as ADC candidates.

ADC Target
ADC Antibody
ADC Linker
ADC payload drug

Name
IgG Fc

Overview
The fragment crystallizable region (Fc region) is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc region of IgG bears a highly conserved N-glycosylation site. Glycosylation of the Fc fragment is essential for Fc receptor-mediated activity. Fc binds to various cell receptors and complement proteins thus mediating different physiological effects of antibodies, such as opsonization, antibody dependent cellular cytotoxicity (ADCC), degranulation of mast cells, basophils, eosinophils and other processes.

Overview
Fab fragment of anti-rat IgG Fc specific polyclonal antibody

Species Reactivity
Rat

Name
Cleavable linkers

Description
Cleavable linkers rely on the physiological stimuli, which mainly include chemically cleavable linkers and enzymatically cleavable linkers. Chemically cleavable linkers including acid-labile linkers and disulfide linkers. For acid-labile linkers, intracellular release of payloads relies on the different pH between endosomes/lysosomes and blood. The release of disulﬁde-linked drugs is controlled by the factors in intracellular environment. Enzymatically cleavable linkers, peptide linkers and β-glucuronide linkers, are sensitive to enzymes located in cytoplasm.

Name
DMDM (duocarmycin DM)

Description
Duocarmycins belong to the minor-groove-binding DNA-alkylating agents (DNA MGBA). The natural duocarmycins are isolated from the culture broth of Streptmyces sp and a series of derivatives has also been synthesized.

For Research Use Only. NOT FOR CLINICAL USE.

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Customer Reviews FAQ

Excellent

Excellent product!

The anti-Rat IgG(Fc)Fab-C-DMDM ADC provided outstanding specificity and reliable cytotoxicity. The cleavable linker ensured precise targeting, and the product showed no toxicity in our tests.

Excellent

Top-notch ADC!

The DMDM conjugate worked perfectly, causing effective DNA damage with minimal background interference. This product has become essential for our monoclonal antibody pre-screening.

Excellent

Highly satisfied!

The anti-Rat IgG(Fc)Fab-C-DMDM ADC delivered consistent results with excellent specificity. The low toxicity profile allowed us to perform sensitive experiments with confidence.

Excellent

Impressive results!

The anti-Rat IgG(Fc)Fab-C-DMDM ADC showed great performance in our assays. The cleavable linker to DMDM provided efficient DNA damage with no significant side effects.

Excellent

Great product!

The anti-Rat IgG(Fc)Fab-C-DMDM ADC exceeded our expectations with its strong cytotoxic effects and low background. The quality assurance and prompt delivery were also much appreciated

Excellent

Highly recommend this ADC!

The anti-Rat IgG(Fc)Fab-C-DMDM ADC provided precise targeting and strong cytotoxicity. The product's safety profile made it an ideal choice for our experiments.

Q: 1: How does the cleavable linker benefit the functionality of the anti-Rat IgG(Fc)Fab-C-DMDM ADC?
A: The cleavable linker in the ADC allows for the controlled release of DMDM upon internalization into target cells. This feature ensures that the cytotoxic agent is activated only within the intended cells, enhancing the specificity of the ADC and reducing potential side effects on non-target cells.
Q: 2: What are the primary research applications of the anti-Rat IgG(Fc)Fab-C-DMDM ADC?
A: This ADC is primarily used for pre-screening rat monoclonal antibodies as potential ADC candidates in research settings. It enables efficient and economical testing of antibodies for their binding efficacy and therapeutic potential against cells expressing rat IgG Fc.
Q: 3: What role does glycosylation play in the functionality of the anti-Rat IgG(Fc)Fab-C-DMDM ADC?
A: Glycosylation at the Fc fragment of the antibody enhances its stability and interaction with Fc receptors on immune cells, which is crucial for mediating immune responses such as ADCC and opsonization. These interactions are vital for the effective functioning of the ADC in biological systems.
Q: 4: What is the significance of the anti-Rat IgG(Fc)Fab-C-DMDM ADC in the development of rat-specific immunotherapies?
A: This ADC plays a crucial role in the development of rat-specific immunotherapies by allowing researchers to screen and validate rat monoclonal antibodies that could be used in targeted therapies. Its ability to specifically bind and deliver cytotoxic agents to cells expressing rat IgG Fc receptors makes it an invaluable tool in designing therapies that are optimized for efficacy and specificity in rat models of disease.
Q: 5: What experimental setups are most suitable for testing the efficacy of the anti-Rat IgG(Fc)Fab-C-DMDM ADC?
A: The ADC's efficacy is best evaluated in cell-based assays that express rat IgG Fc receptors. These setups can include in vitro cytotoxicity assays, binding affinity tests, and potentially in vivo models designed to assess targeted drug delivery and therapeutic outcomes.

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Same Target Same Linker Same Payload

CAT#	Product Name	Linker	Payload
ADC-AA-050	Protein G-MCC-DM1 ADC	MCC (Maleimidomethyl cyclohexane-1-carboxylate)	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)
ADC-AA-053	Protein A-MMAF ADC		MMAF (Monomethyl auristatin F)
ADC-AA-027	anti-MIgG(Fc)Fab-N-MMAF ADC	Noncleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-028	anti-MIgG(Fc)Fab-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-026	anti-MIgG(Fc)-N-DM1 ADC	Noncleavable linkers	DM1 (N2’-Deacetyl-N2’-(3-mercapto-1-oxopropyl)maytansine)

CAT#	Product Name	Linker	Payload
ADC-AA-066	Anti-Rat IgG(H+L)-C-PBD ADC	Cleavable linkers	PBD (pyrrolobenzodiazepine)
ADC-AA-002	anti-HIgG(Fc)-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-010	anti-HIgG(Fc)Fab-C-MMAF ADC	Cleavable linkers	MMAF (Monomethyl auristatin F)
ADC-AA-017	anti-HIgG(Fab)-C-MMAE ADC	Cleavable linkers	MMAE (Monomethyl auristatin E)
ADC-AA-021	anti-MIgG(Fc)-C-MMAE ADC	Cleavable linkers	MMAE (Monomethyl auristatin E)

CAT#	Product Name	Linker	Payload
ADC-AA-006	anti-HIgG(Fc)-C-DMDM ADC	Cleavable linkers	DMDM (duocarmycin DM)
ADC-AA-024	anti-MIgG(Fc)-C-DMDM ADC	Cleavable linkers	DMDM (duocarmycin DM)
ADC-AA-013	anti-HIgG(Fc)Fab-C-DMDM ADC	Cleavable linkers	DMDM (duocarmycin DM)
ADC-AA-030	anti-MIgG(Fc)Fab-C-DMDM ADC	Cleavable linkers	DMDM (duocarmycin DM)

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