Agonistic Monoclonal Antibody Service

With the development of antibody-production techniques, a number of antibodies have been developed for multiple applications, such as research, diagnostic and therapeutic. As a leading service provider focusing on antibody engineering, Creative Biolabs offers high-quality antibody engineering service to promote the development of agonistic monoclonal antibody.

Different from the initial mechanism to harness the actions exerted by MAbs, such as neutralization, blocking, ADCC, CDC, agonistic monoclonal antibody functions as agonists of crucial receptors, essentially activating receptors and amplifying immune responses. The agonist activity can occur when the antibody binds the receptor in a manner that mimics the binding of the natural ligand, resulting in antibody-mediated downstream signaling or agonism. Recently, numerous agonistic monoclonal antibodies emerge as one effective way to boost immune responses against specific targets to fight cancer. Particularly, we are capable of offering a full package of agonistic monoclonal antibody engineering services listed as below.

Fig.1 Mechanisms of receptor supercluster formation with co-stimulatory agonists. (Mayes, 2018)

Custom Monoclonal Antibody Production by Hybridoma Technology

Our antibody production services include the custom monoclonal antibody generation, from gene expression or peptide synthesis to antibody purification and labeling. With our guarantee, we can produce antibodies in either research quantities or on large scale for industry application. Importantly, we can produce monoclonal antibodies within 10 weeks.

Chimeric (Recombinant) Antibody Construction

Creative Biolabs has developed a proprietary procedure for Hi-Affi™ recombinant antibody production, with optimized library construction and screening processes using the animal-free system. We guarantee successful isolation of clones in a short time with specific expression and characterization requirements. In addition, orders ranging from 1mg to 10g is feasible.

Antibody Humanization and Affinity Maturation

We offer a proprietary antibody humanization service incorporated with affinity maturation. Using our advanced technologies, there is no need to improve the affinity after humanization. In addition, we also provide humanization service to antibodies derived from other species, such as non-human primate (NHP), rabbit, dog, chicken, llama.

Antibody Epitope Mapping

Based on advanced antibody epitope mapping platform, we are able to map the epitopes of a large variety of antibody forms, including intact IgG, Fab, scFv, F(ab')2, diabody, minibody, miniantibody, tandem scFv, and single domain antibody as well as protein A scaffold.

Phage Display Library Screening

Our scientists have extensive experience in the screening of phage display libraries. In general, by conducting four rounds of library screening, we can select scFv/Fab antibodies with the affinity of 10⁻⁷. Through constructing serial sub-libraries of the isolated scFv/Fab antibodies, we can increase the affinity from 10⁻⁸ to 10⁻⁹. Moreover, we have successfully obtained a scFv antibody with an extremely high affinity of 10⁻¹².

Creative Biolabs is devoted to offering agonistic monoclonal antibody engineering services as a stand-alone service or part of a complete antibody humanization package for global customers. With years of experience, our professional scientists are happy to drive your research from any stage to final production of 100% royalty-free humanized antibodies. For more detailed information, please feel free to contact us.

Reference

Mayes, P.A., et al. The promise and challenges of immune agonist antibody development in cancer. Nature reviews Drug discovery. 2018, 17(7), p.509.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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