Protein Expression in Bacteria

Bacterial Expression Systems (E. coli / Bacillus)

Over the years, Creative Biolabs has developed BacTEC and FoldEZ™ technologies to tackle hard-to-express and hard-to-dissolve proteins. With BacTEC™ technology, the expression level can be greatly improved for almost all of the proteins. In addition, over 98% of the inclusion bodies can be refolded and solubilized by our proprietary FoldEZ™ protein refolding technology.
Our OxiCyto™ competent E.coli strains are excellent options for expressing proteins having complex disulfide bonds.

E. coli Expression

Protein Expression in Bacteria

The first choice for the expression of recombinant proteins is typically E. coli. Production of proteins in this bacterial strain is well-established, fast and simple and usually provides high yields. Recent progress in the fundamental understanding of transcription, translation, and protein folding in E. coli, improved genetic tools had made this bacterium more valuable than ever for the expression of complex eukaryotic proteins.

Creative Biolabs' newly-built facilities supporting a vast array of state-of-the-art equipment are leveraged by our experienced PhD-level scientists to deliver proteins that require glycosylation modifications to be expressed via microbial systems quickly and economically.

One of the most popular technologies is BacTEC™ system. Creative Biolabs' patented BacTEC™ system is capable of achieving soluble expression levels well over 20 g/L. While the technology has shown it can express many types of proteins, it has established a track record for producing high-quality, correctly folded antibody fragments (including Fabs and scFv).

Our FoldEZ™ protein refolding technology is designed to determine the optimal refolding conditions by evaluation a series of buffers. We provide refolded protein at purity of 95% or greater.

Exclusively from Creative Biolabs, OxiCyto™ competent E.coli strains are engineered to enable the folding of recombinantly expressed proteins in vivo. The expression of the isomerase, DsbC, within the OxiCyto cytoplasm enhances the correct formation of disulfide bonds in proteins that require them for biological activity.

B. subtilis Expression

Bacillus subtilis is a significant microorganism in the scientific research field. It has been developed as an attractive host for the production of recombinant proteins. Our Bacillus subtilis system is an ideal option for the expression of monomeric protein products at expression levels up to 20g/L. This high yield, combined with our patented fermentation protocols allows for fast process development and scale-up to support early clinical testing.

Bacterial Expression Systems (E. coli / Bacillus)

All listed customized services & products are for research use only, not intended for pharmaceutical, diagnostic, therapeutic or any in vivo human use.

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