The most basic function of an antibody is to neutralize the target antigen, and other functions include cross-linking to induce soluble antigen precipitation or cell agglutination. Antibodies are one of the most commonly used tools in basic scientific research, in vitro diagnosis, and clinical testing. They were routinely used for Western blotting (WB), quantitative immunofluorescence (QIF), enzyme-linked immunosorbent assay (ELISA), immunoprecipitation (IP), and immunohistochemistry (IHC). They are also important tools for clinical management, widely used in laboratory medicine (ELISA assay and flow cytometry) and anatomical pathology (IHC). The significant impact of antibody therapy in disease treatment has led to the emergence of many new forms of macromolecules. Antibodies and antibody-derived macromolecules have become the main pillars of protein-based therapeutic molecules (biologics), which are widely used in the treatment of various diseases and disease states, including cardiovascular diseases, autoimmune diseases, malignant tumors, and infections.
Specific antibodies are widely used in the biomedical field, and currently, the screening methods for specific antibodies mainly include hybridoma technology and antibody engineering technology. However, these techniques have become the bottleneck of specific antibody screening due to their complicated screening process, long preparation cycle, high cost, low efficiency, and high automation. To overcome these difficulties, high-throughput antibody expression technology has become a promising technology for high-throughput screening of high-purity antibodies.
Fig.1 General workflow of HT antibody production.
Affinity assay
Antibody characterization
The applications of high-throughput antibody expression and purification fall into two broad classes. For microscale applications, HT antibody purification provides a front-end for antibody production to meet various applications, such as analysis of protein interactions, enzyme substrate networks, etc., with limited antibody quality required for each sample. For applications that require a large amount of purified antibody (mg), the HT method for protein production can effectively screen for many different constructs to identify those candidates that have high activity and better characters. In these applications, success often depends on the time-consuming trial-and-error process of attempting to express different versions of the target protein until a well-expressing, soluble, and correctly folded construct can be identified. The ability to screen multiple structures simultaneously can greatly speed up the screening process.
Fig.2 Applications for HT protein purification.
Whole proteome antibody sets will be valuable research resources and can be used for highly parallel detection, such as microarrays. Numerous attractive possibilities currently exist for HT protein production efforts. The increase in speed and throughput of our workflow enables researchers to easily and quickly screen a large number of antibodies, thereby promoting the downward selection of efficient candidate antibodies. Our method will contribute to the development of antibodies for therapeutic, diagnostic, and research applications.
Creative Biolabs' scientists have been committed to building an advanced technology platform in the field of antibodies, and strive to improve the efficiency based on improving the quality of products. With our high-throughput antibody expression platform, we can enhance a range of purified antibody products for customers, helping to accelerate therapeutic development and academic research. If you have any interest in our platform service, please don't hesitate to contact us.
Welcome! For price inquiries, we will get back to you as soon as possible.
INQUIRY
SERVICES
PRODUCTS
PLATFORMS
