Bispecific antibodies (BsAbs) recognize two different epitopes, based on the coupling of two antigen-recognizing elements into a single construct. During the development of BsAbs, biochemical characterization serves as an essential part, both for basic and clinical research. With years of study, Creative Biolabs has established a comprehensive biochemical characterization platform that enables rapid measurement of the Purity, Molecular Weight/Mass, and Molecular Structure of BsAbs. Based on our well-established platform, we provide best-in-field custom biochemical characterization services to achieve your goal.
Antibody-purity analysis is very important for the successful development of BsAbs based biopharmaceuticals. We offer the sodium-dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis, which is a classical technique for antibody-purity analysis. Besides, we provide the liquid chromatography-mass spectrometry (LC-MS) analysis and partial-filling affinity capillary electrophoresis (PFACE) analysis, allowing more accurate qualitative and quantitative analysis of the antibody.
The development of a fast and precise method to measure the molecular weight of BsAbs is a great challenge in the field of therapeutic antibodies. Creative biolabs offers a variety of techniques for the determination of exact molecular weight of BsAbs, including size-exclusion chromatography multi-angle light scattering (SEC-MALS), electrospray ionization mass spectrometry (ESI-MS) and electrospray ionization time-of-flight (ESI-TOF). SEC-MALS is a powerful technique to measure masses of macromolecules. The ESI-MS approach allows the ionization of large biological molecules (such as proteins) without them being fragmented. With these advanced techniques, we can determine the exact molecular weight of your antibody either in their native form or in their fragmented form.
BsAbs are divided into two classes: those with a Fc region and those without a Fc region. The development of recombinant DNA technology has led to a variety of recombinant BsAbs with over 50 different formats now being produced. To study the interactions between antibodies with ligands, high-resolution structures of the antibodies are required. The structural information is extremely important in the engineering of antibodies for therapeutic and other purposes. Creative Biolabs provides various methods for molecular structure analysis of BsAbs, including nuclear magnetic resonance (NMR) spectroscopy, small-angle X-ray scattering (SAXS), and molecular docking analysis. NMR spectroscopy is a powerful and theoretically complex analytical tool. SAXS is a useful technique to study the sizes of a variety of particle, from small peptides to large macromolecular machineries. Molecular docking predicts the preferred orientation of one molecule to another when bound to each other to produce a stable complex.
Figure 1. Structures of Anti-ZIKV Fabs and scFv Complexed with DIII (Zhao, H. 2016).
With the latest instruments and professional staff, Creative Biolabs is dedicated to offering first-class biochemical characterization services to meet your goal of BsAb development in a time-saving manner. Creative Biolabs also provides various other services related to BsAbs development. Please feel free to contact us for more information and a detailed quote.
1. Zhao, H.; et al. Structural basis of Zika virus-specific antibody protection. Cell. 2016, 166(4): 1016-1027.
2. Hura, G. L.; et al. Robust, high-throughput solution structural analyses by small angle X-ray scattering (SAXS). Nature methods. 2009, 6(8): 606-612.