More and more attention has been paid to investigate the roles of exosomes in therapeutic and diagnostic applications. Exosome labeling is a novel strategy for monitoring exosomes trafficking in vivo, which contributes to optimizing exosome-based diagnosis and treatment. With years of experiences in exosome labeling, Creative Biolabs offers high-quality exosome fluorescent labeling services to satisfy our customer’s requirements.

Fluorescent Labeling

Fluorescent labeling uses a reactive derivative of a fluorescent molecule known as a fluorophore which selectively binds to a specific region or functional group on the target molecule and can be attached chemically or biologically. Ethidium bromide, fluorescein and green fluorescent protein are common labels. The most commonly labeled molecules are antibodies, proteins, amino acids and peptides which are then used as specific probes for detection of a particular target. Fluorescein labeling technique has been extended over a large area of cell biology, and a variety of fluorescent protein-derived techniques have been developed to visualize the functions and conditions of the molecules within living cells.

Fig.1 Excitation and emission of a fluorophore. (Toseland, 2013)

Exosomes are a kind of extracellular vesicles with 30-150 nm diameter and cargo many components of origin cells such as proteins and nucleic acids. Fluorescent labeling provides a powerful tool for the investigation of biological features of exosome. For example, total exosome can be labeled by fluorescent dyes, which can be used to detect the distribution and location as well as trafficking of exosomes. Besides, antibodies labeled by fluorescent dyes have a high affinity with specific antigens on the exosomes. Hence, antibody fluorescent labeling allows us to monitor characteristics of specific exosomes in vivo.

Fig.2 Confocal microscopy- Green Fluorescent labeled exosomes. (Momen-Heravi, F. et al., 2015)

Application of Exosome Fluorescent Labeling

• Identification of Exosome Characteristics—Fluorescence conjugated antibody allows to visualize and recognize individual exosomes by a confocal laser scanning microscope or flow cytometry. This technology helps identify the distribution and concentration of the interested exosomes, making it a promising source for diagnostics.
• Tracking Exosome in Vivo—exosome can be used as a vector for drug delivery to target tissue. Fluorescence labeling makes it possible to visualize and monitor the movement of exosomes bearing drugs in vivo, providing an effective tool for the optimization of treatment protocols in time.

Advantages and Features of Exosome Fluorescent Labeling Service in Creative Biolabs

• The highest sensitivity and specificity
• Low effect on exosome features
• A high range of flexibility in dye labeling
• All-around technical service

Exosome fluorescent labeling in Creative Biolabs is an effective tool for the investigation of exosomes characteristics and exosomes trafficking for the therapeutic and diagnostic application. If you are interested in exosome labeling services, please contact us for more information whenever you feel free.

References:

1. Toseland, C. P. Fluorescent labeling and modification of proteins. Journal of Chemical Biology. 2013, 6(3):85-95.
2. Momen-Heravi F,; et al. Exosomes derived from alcohol-treated hepatocytes horizontally transfer liver specific miRNA-122 and sensitize monocytes to LPS. Scientific reports. 2015, 5: 9991.

• Exosome Biotinylation