In the recent years, more and more attention has been paid to investigate the roles of exosomes in therapeutic and diagnostic applications. Exosome labeling is a novel strategy for monitoring exosomes trafficking in vivo, which contributes to optimizing exosome-based diagnosis and treatment. With years of experiences in exosome labeling, Creative Biolabs has developed fluorescent labeling and biotin labeling techniques to track the distribution and movement of the specific exosomes.
Exosomes are small vesicles (30-150 nm in diameter) containing sophisticated RNA and protein cargos and function as a vector of intercellular communication. Up to now, they have been of interest to the scientific community for the roles they play in physiological and pathological processes and their potential for therapeutic and diagnostic applications. In order to further our understandings of exosome characteristics, exosome labeling strategy is raised to investigate the biology and distribution of exosomes, and monitor exosomes trafficking in real time in vivo without invasion. This method is an effective tool for the development and optimization of exosome-based diagnosis and treatment.
Fig.1 Detection of extracellular vesicles by flow cytometry with calcein AM. (Gray, 2015)
The investigation of exosomes biodistribution, as well as their tracking, delivery, and tumor targeting via exosome labeling make it possible to determine their physiological functions and to exploit imaging-derived information for exosome-based theranostics. Here, we offer two technological platforms for exosomes labeling.
Antibodies labeled with fluorescent dyes have high affinity with the specific cell-of-origin antigens on the surface of exosomes. And fluorescent dyes can directly label proteins on exosomes. Hence, exosomes labeled with fluorescent dyes can be used to visualize and track specific components in isolated exosomes or to monitor exosome cargo delivery to cells in real-time.
Biotin is a small molecule that forms one of the strongest non-covalent interactions found in nature with its binding partners, avidin, and streptavidin. The high tight non-covalent bond makes labeling proteins with biotin a useful tool for application such as exosome purification and detection. For example, exosomes bearing specific proteins labeled with biotin are used to detect exosome trafficking in vivo or model animal. Importantly, due to its size, biotin rarely disrupts the activity of proteins, making it a good choice for a label.
Exosome labeling in Creative Biolabs acts as an effective tool for the investigation of exosomes characteristics and exosomes trafficking for the therapeutic and diagnostic application. If you are interested in exosome labeling, please contact us for more information whenever you feel free.