Plant-derived Exosome Isolation and Development Services

Creative Biolabs has launched an innovative customized production service for plant-derived exosomes, capable of providing pilot study services for short-batch isolation and expanding to large-scale manufacturing development services.

Introduction to Major Types of Plant-derived Exosomes

Fruit-derived exosomes

Mainly found in the pericarp, pulp, and other parts of the fruit
Their generation is regulated by plant growth stage, environmental conditions, and other factors.
Rich in antioxidants, vitamins, hormones, signaling molecules, and other substances, with antibacterial, antioxidant, promote cell proliferation and immune regulation, and other biological activities.

Vegetable-derived exosomes

Generally found in tissues such as leaves, roots, and seeds.
Rich in nutrients, such as vitamins, alkaloids, dietary fiber, etc. as well as various bioactive substances, such as antioxidants, anticancer substances, and immunomodulatory substances.
Possess positive effects on human health in multiple ways, such as the prevention of chronic diseases, improvement of digestive system function, and regulation of the immune system.

Medicinal plant-derived exosomes

Mainly distributed in roots, stems, leaves, and other parts.
Rich in active ingredients, such as alkaloids, flavonoids, saponins, volatile oils, and so on.
With pharmacological activity and biological activity, exosomes are not only an important source of extracted active ingredients, but also of significance to the in-depth understanding of the pharmacological effects and mechanisms of medicinal plants.

Fig. 1 Sources, biogenesis, and contents of plant-derived exosomes.¹

Plant Exosome Production Services

At Creative Biolabs, plant exosome production services start with pilot studies of exosome isolation and functional validation from small batches of plant samples, and if the pilot results meet client's expectations, further expansion to large-scale manufacturing development, or even GMP manufacturing, is possible.

Pilot Study

PART 1 Exosome isolation from small batches of plants

Plant sample preparation: pre-treated samples of native plants provided by the customer or specified plant types provided by the supplier
Plant exosome isolation: by pretreatment + filtration and concentration + ultracentrifugation
Plant exosome characterization: detection of particle size and morphology (by NTA and TEM)
Cell experiments:
- Cellular uptake of exosomes assay
- Cell migration assay
- Cell scratching assay

PART 2 Exosome quality control

Mycoplasma assay (By PCR).
Sterility assay, PCR assay to identify bacteria, and sequencing to identify fungi.
Endotoxin assay (By Gel Clot LAL).
Endotoxin removal. Perform endotoxin removal to a limited sensitivity value for single samples in single runs.

PART 3 Animal experiments

Rat skin wound modeling.
Exosome administration.
Wound healing assay.
H&E histology analysis.
Routine pathological analysis.

If pilot study results meet requirements, then expand to large-scale manufacturing.

Determine output rates
Scientific grade manufacturing / GMP grade manufacturing.

Applications

There have been numerous precursor studies on the potential of plant exosomes for disease treatment and skin care.

Disease alleviation

1. Regulate cellular homeostasis

Ginger exosomes have excellent hepatoprotective effects, acting on the Nrf2 pathway to direct hepatic detoxification and enhance antioxidant gene expression.

2. Moderate tumor progression

Citrus exosome administration exerted a strong tumor growth inhibitory effect in an in vivo xenograft model of chronic granulocytic leukemia.

3. Promote tissue repair

Grape-derived exosomes promoted intestinal tissue repair, targeted intestinal stem cells and induced their proliferation, and upregulated pluripotent stem cell markers.

Skin Care

1. Promote transdermal absorption

An in vitro study of broccoli exosomes found that they have strong stratum corneum penetration ability, and are able to encapsulate fluorescent molecules for better penetration and distribution in the stratum corneum and dermis.

2. Drug slow-release system

Plant exosomes are suitable for loading astaxanthin, retinol, and other molecules that require wrapping treatment to achieve slow drug release.

3. Regulates hyperpigmentation and anti-aging

Ginseng exosomes ameliorate the senescence phenotype of human skin fibroblasts by down-regulating senescence-associated molecules and melanin formation-associated proteins, as well as the pigmentation phenotype associated with the senescence of human melanocytes under UV radiation.

Fig. 2 Plant-derived exosome biofunctions and their applications.²

Creative Biolabs' well-established platform for customized isolation and development of plant exosomes for almost types is a reliable tool to assist you in advancing the plant exosome research you desire. Please inquire us to get a customized solution.

References

Sarasati, Andari, et al. "Plant-Derived Exosome-like Nanoparticles for Biomedical Applications and Regenerative Therapy." Biomedicines 11.4 (2023): 1053.
Dad, Haseeb Anwar, et al. "Plant exosome-like nanovesicles: emerging therapeutics and drug delivery nanoplatforms." Molecular Therapy 29.1 (2021): 13-31.

Plant Exosome Isolation