Serum/Plasma derived Exosome Isolation & Development Service

Overview Workflow Highlights Advantages Testimonials FAQs

Overview

Creative Biolabs specializes in isolating and developing exosomes from human serum and plasma, emphasizing high-quality preparations suitable for biomarker discovery and mechanistic research. Our workflows are optimized to maintain vesicle integrity and reduce protein or lipoprotein contamination, allowing researchers to obtain material that supports reliable downstream data generation. We also accept samples from other species when required and maintain consistent SOPs for larger cohort studies.

Using Size-Exclusion Chromatography (SEC) as our primary technology, Creative Biolabs delivers high-purity extracellular vesicles that retain native biological properties. SEC is particularly advantageous for serum/plasma due to its ability to remove abundant soluble proteins without imposing shear stress. For projects requiring unique vesicle populations, we further offer affinity-based or antibody-assisted enrichment tailored to specific biological questions.

Isolation & Development Workflow

Sample Handling

Guidance on minimal required volume.
Evaluation of sample integrity, hemolysis level, and anticoagulant compatibility.
Optional pre-processing (centrifugation/filtration) performed by Creative Biolabs.

SEC-Based Isolation (Creative Biolabs Standard)

Fractionation through pharmaceutical-grade SEC columns.
High selectivity against lipoproteins and abundant serum proteins.
Optimized elution windows to maximize vesicle purity without compromising yield.

Targeted EV Capture (Optional)

High-specificity affinity matrices for isolating niche vesicle populations.
Ideal for cancer biomarker studies or cell-type-specific investigations.
Compatible with downstream proteomics and RNA assays.

Concentration & Buffer Exchange

Ultrafiltration with molecular-weight cutoffs tailored to EV size ranges.
Low-pressure handling to maintain vesicle integrity.
Delivery in PBS, EV-free buffer, or client-requested formulations.

Exosome Development Workstreams

Coating, labeling, or surface marker enhancement for functional assays.
Optional enrichment for small RNA–rich or protein-rich EV subsets.
Multi-sample batch processing for longitudinal or cohort-based studies.

If you would like Creative Biolabs to plan a complete workflow for your study, feel free to contact our scientific team.

Fig.1 SEC is utilized to isolate exosomes from samples of biological fluids. Fig.1 Exosomes are separated from biological fluid samples using SEC.¹

Research Highlights

Biomarker Discovery Potential

Serum/plasma EVs provide rich molecular signatures reflective of systemic physiology.
Excellent matrices for identifying RNA, protein, or lipid markers.
Suitable for early-stage hypothesis generation in disease research.

Multi-Omics Compatibility

High-purity SEC fractions allow:

miRNA sequencing and small RNA-seq.
Proteomics (DDA/DIA), phosphoproteomics, and EV-surface profiling.
Lipidomics and metabolomics.

Creative Biolabs ensures reagent and buffer compatibility to facilitate cross-platform integration.

Quality & Reproducibility

All protocols validated for low variability across replicate isolations.
Calibrated against NTA, protein assays, and EV marker expression.
Optimization available for low-abundance biomarker studies.

Creative Biolabs' scientists would be glad to help design a biomarker discovery strategy aligned with your research.

Key Advantages of Creative Biolabs

SEC-Centered Purification Excellence

Minimal contamination by albumin, apolipoproteins, and soluble plasma proteins.
Reliable vesicle size profile and particle integrity.
Especially suitable for sensitive downstream molecular analyses.

Highly Customizable Isolation Menu

Flexibility to choose methods based on purity, yield, or target vesicle type.
Ability to combine SEC + affinity for enhanced specificity.
Project-specific workflow recommendations provided by Creative Biolabs scientists.

Complete Analytical Ecosystem

Characterization services include:
- TEM/cry-TEM, NTA, DLS for physical properties.
- Western blot/ELISA/flow for tetraspanin and cargo markers.
- RNA, proteomic, metabolomic, and lipidomic profiling pipelines.
Optional data interpretation support tailored to biological questions.

High-Value Exosome Isolation Kits

Cost-effective SEC-based kits designed for easy operation in individual labs.
Rapid isolation (30–40 min) with consistent recovery.
Suitable for teams needing scalable or in-house workflows.

If you'd like help selecting the most suitable service or kit, Creative Biolabs' team is available for consultation.

Client Feedback

"Creative Biolabs has emerged as our favored collaborator for projects involving serum extracellular vesicles. Their SEC workflow consistently delivered highly clean vesicle fractions, and the miRNA sequencing results were some of the most robust we have obtained."

"Our biomarker discovery initiative greatly benefited from the valuable scientific insights provided by Creative Biolabs. Their team did not simply run samples—they helped guide the design, offered clear recommendations, and provided detailed QC data that made interpretation straightforward."

"The thoroughness and clarity of their data packages facilitated a seamless peer review process, and our collaborators noted the dependability of the results."

To experience the same level of research partnership, Creative Biolabs invites you to speak with our team.

Creative Biolabs is committed to building dependable and scientifically rigorous exosome solutions for serum and plasma research. Whether you are exploring novel biomarkers, validating hypotheses, or mapping molecular cargo profiles, our team offers the expertise and infrastructure necessary to drive meaningful discoveries. We welcome collaborations of all sizes and look forward to supporting your next research milestone. For a customized quotation or consultation, Creative Biolabs invites you to contact our specialists anytime.

FAQs

Q: Do you accept low-volume samples?

A: Yes. Creative Biolabs can isolate exosomes from as little as 100–200 µL, although 1-5 mL is recommended for optimal multi-omics output. We provide guidance on adjusting workflows when researchers need to work with particularly scarce materials.

Q: Can SEC reduce lipoprotein contamination in plasma samples?

A: Yes. Creative Biolabs uses validated SEC matrices and optimized collection windows that substantially minimize LDL/HDL carryover. For studies that require even higher specificity, we can incorporate additional enrichment steps.

Q: What downstream analyses are available?

A: Creative Biolabs supports miRNA sequencing, total RNA sequencing, proteomics, lipidomics, TEM imaging, NTA, Western blotting, and EV-flow profiling. We can combine multiple analyses to support multilayer biomarker discovery.

Q: Do you support larger cohort studies?

A: Absolutely. Creative Biolabs has standardized workflows capable of handling dozens to hundreds of samples with reproducible performance. Batch-to-batch consistency is ensured through rigorous QC checkpoints.

Reference

Dilsiz, Nihat. "A comprehensive review on recent advances in exosome isolation and characterization: Toward clinical applications." Translational oncology vol. 50 (2024): 102121. https://doi.org/10.1016/j.tranon.2024.102121. Distributed under Open Access license CC BY 4.0. The image was modified by revising the title.