Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsCreative Biolabs provides highly specific and sensitive anti-formylated protein antibodies based on our excellent High-Affi™ technology.
Formylation is an identified post-translational modification (PTM) on lysine that's reversible, dynamic, and found everywhere from bacteria to eukaryotes. Mass spectrometry analysis shows that, in core histones, this formylation pops up on lysine residues that we already know are key for organizing nucleosomes, like Lys5, Lys116, and Lys120 on H2B, and Lys91 on H4. Then, in linker histones, they've mapped several formylation sites that play a big role in DNA binding. Given the significant abundance of lysine formylation in chromosomal proteins, it's pretty likely that it plays a part in the epigenetic stuff that governs how chromatin works.
Interestingly, formylation can also show up from oxidative DNA damage. When cells are stressed by oxidation, formylation can actually outcompete acetylation on lysine because formylphosphate is so reactive. Histone formylation can really jump up under this stress. So, it looks like lysine formylation is important for understanding chromatin regulation – things like DNA binding, repair, condensation, and epigenetics. Plus, if it goes dysregulation, it might lead to diseases like cancer.
Fig.1 Formylation of lysine can compete with acetylation. Distribute under CC BY-SA 3.0, from Wiki, without modification.
For studying PTMs, especially finding modification sites and new types, antibodies are a must. Creative Biolabs has years of experience discovering and producing them. We've got Ph.D. scientists focused on PTM research, from making antibodies to analyzing modifications. Our custom antibodies have high affinity and specificity and don't react with peptides that have similar modifications like mono-, di-methylation, acetylation, and other acylations.
Polyclonal Antibodies: These are a mix of antibodies produced by different immune cells in an animal, all recognizing various parts of a formylated peptide. They can be generated relatively quickly and offer strong binding.
Monoclonal Antibodies: These are highly specific antibodies produced by identical immune cells, each targeting a single site on the formylated lysine (ideally within a specific sequence). They offer consistent performance and high specificity.
Site-Specific Anti-formylation Antibodies: These highly specific antibodies are meticulously designed to recognize and bind exclusively to formylated lysine residues at particular locations on a target protein. This precise targeting allows for the unambiguous identification and quantification of site-specific formylation events, crucial for dissecting the functional consequences of this modification at a granular level.
Pan Anti-formylation Antibodies: These offer a broad approach to detect and quantify protein formylation. These antibodies recognize formylated lysine residues regardless of their position within a protein sequence. They are valuable tools for surveying the overall level of protein formylation across complex biological samples.
Making really specific polyclonal antibodies against formylated lysine usually means injecting an animal with a custom peptide that's got the formyl group on it. The animal's blood then has a mix of antibodies, some good at sticking to our target. To get only those, we use a special column that grabs the formylated peptide. We wash away the rest and collect the pure antibodies. Extra steps can ensure they only bind to the formylated version. This way is pretty quick, and the mix of antibodies can be really good at grabbing their target.
Phage display technology enables the screening of diverse antibody repertoires to identify those with high affinity for formylated peptide. This technique uses phage libraries created by immunizing animals, leveraging their immune response for specific antibodies. Alternatively, ready-made libraries from non-immune animals or human sources can be used. These offer a broad range of antibodies for selection. Through repeated binding and washing steps against the target, phages displaying strong binders are concentrated, enabling the isolation and characterization of monoclonal antibodies.
The hybridoma method starts by getting an animal's immune system to make anti-formylation antibodies. We fuse their antibody-making cells with immortal cancer cells to create hybridomas that both make antibodies and live forever. We then find the hybridomas making the right specific antibodies and grow them to get a pure cell line that keeps producing them. This often gives us really good antibodies refined by the animal's immune system.
Based on advanced platforms and rich experience in antibody development, Creative Biolabs can provide comprehensive PTM-specific antibody production services of your choice.
Custom Formylated Peptide Design: We expertly design and synthesize high-quality formylated peptides tailored to your specific targets, ensuring optimal presentation of the formyl modification for antibody generation.
Stringent Specificity Screening: Our rigorous testing guarantees high specificity for the formyl group on lysine, with minimal cross-reactivity against other common lysine modifications.
Multiple Antibody Platforms: We offer hybridoma and phage display technologies optimized for selecting high-affinity and specific antibodies against formylated epitopes, allowing a tailored approach.
Context-Specific Antibody Expertise: We have experience developing antibodies that specifically recognize formylation within particular sequence contexts.
Comprehensive Formylation Characterization: We provide thorough antibody characterization, including affinity and detailed specificity analysis to confirm formyl group recognition.
Monospecific Anti-Formylation Polyclonal Antibody Production
Q: How do you guarantee formylation specificity over other modifications?
A: Our rigorous screening includes testing against unmodified and other modified lysines (acetylation, methylation, etc.). We provide data demonstrating minimal cross-reactivity, ensuring high specificity for the formyl group through stringent quality control.
Q: What peptide design is crucial for good formylation antibodies?
A: We carefully design the peptide sequence around the formylated lysine to optimize its presentation for antibody generation, considering flanking residues and conjugation to enhance recognition of the formyl modification.
Q: What are the timelines and deliverables for antibody discovery?
A: Polyclonal projects take 4-5 months; monoclonal, 5-7 months. Deliverables include purified antibodies, characterization data (specificity, affinity), and clone/production info. We provide regular updates.
Q: What challenges arise with novel PTM antibodies like formylation?
A: The small formyl group can be a weak trigger for the immune system or selection. We use strategic peptide design, conjugation, and optimized screening to address this. Distinguishing it from similar modifications is tackled with thorough specificity testing.
Q: Can you target formylation on a specific protein or lysine?
A: Yes, our services are customizable. We design immunogens/antigens targeting formylation on your protein and even specific lysines, if the surrounding sequence allows for the necessary specificity.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
