Creative Biolabs provides excellent services for the construction and screening of immune antibody library generated by our advanced phage display technology. Our professional scientists can generate antibody libraries from diverse species, including common laboratory animals [e.g. rodents, rabbit, chicken, dog and monkey (NHP)], and some infrequent antibody sources such as human, camel, shark, bovine, alligator, and so on.
Over the past decades, phage display has been proven to be a powerful technology to ‘display’ millions or even billions of different peptides or proteins. It is now a common choice for the studies of protein-protein, protein-peptide and protein-DNA interactions. The technique is mainly based on the using of bacteriophages, such as T4, T7 and the most common M13 filamentous phage. By fusing a protein encoding gene with bacteriophage coat protein gene, the interested protein can be displayed on the phage surface, which can be then used to detect its interaction or binding ability with other molecules. One of the successful applications of phage display technology is the isolation of monoclonal antibodies using large capacity phage antibody libraries.
Currently, constructing and screening immune antibody library via phage display technology have become a major choice for the production of monoclonal antibody with high specificity and affinity. Compared with the conventional hybridoma technology, the generation of immune antibody libraries is not limited by the requirement of fusion partners, which expands the possibility to develop monoclonal antibodies from a much broader range of species. In general, the phage-displayed immune antibody libraries are constructed in scFv and Fab format, and the antibody gene repertoire is created from the immunoglobulin gene of either spleen B cells (for immunized animals) or peripheral blood B cells (for immunized donors). Theoretically, on the basis of appropriate genome information, phage display technology has potential to construct antibody libraries for every species and develop the corresponding monoclonal antibodies.
At Creative Biolabs, we have succeeded in generating monoclonal antibodies from various host species, including but not limited to:
Creative Biolabs has years of experience in the field of antibody development and phage display technology. Our scientists are confident in generating antibody libraries with a diversity of 108-10, which can meet the needs of the vast majority of research. Meanwhile, we also have extensive experience in designing the most appropriate biopanning strategy to select reliable antibodies with high specificity and affinity. In addition to the regular antibody libraries of experimental animals, our scientists are also able to generate a series of unique antibody libraries from other species, such as human, guinea pig, shark and alligator. As one of the well-recognized industry leaders, Creative Biolabs is committed to offering the most comprehensive antibody production services and providing the best service with high efficiency and quality.