"Creative Biolabs is committed to providing highly customized comprehensive solutions with the best quality to advance our global clients’ projects."
Mammalian Cell based Protein Expression Service
Protein scientists at Creative Biolabs have experience and expertise in both transient and stable expression using a multitude of cell lines, including CHO and HEK293 expression systems. We can enable the generation of high-yielding production systems across different relevant platforms.
Mammalian Cells Expression System
Mammalian cells have the ability to perform the most comprehensive post-translational modifications and to secrete glycoproteins that are correctly folded and contain complex antennary oligosaccharides with terminal sialic acid. Mammalian cells are widely used in the production of recombinant proteins, antibodies, viruses, viral-subunit proteins, and gene therapy vectors.
-
HEK293 expression systems: HEK293 cells produce native structures and biologically functional proteins with similar post-translational modification to the human body. We provide one-stop-shop protein production services using transient transfection in HEK293 cells from gene sequence to purified proteins.
-
CHO expression systems: CHO cells are the most commonly used mammalian cells for protein expression. Our homogeneous stable super-producer clones are obtained without cloning in just one week, whilst the classical generation of stable cell lines takes 6 to 9 months.
Case Studies of Our Mammalian Cell based Protein Expression Service
-
Case 1
HEK293 cells were transfected with the recombinant plasmid containing the target protein and human Fc tag. After cell disruption, WB detection results showed that the target protein was in the secretion supernatant. After affinity purification using a protein A column, a high-purity target protein was obtained.
-
Case 2
The expression vector containing the target protein and His tag was transfected into HEK293 cells. After expression identification, the target protein was in the cell supernatant, and the cell supernatant was Ni-affinity purified to obtain a high-purity target protein.
Fig.1. The SDS-PAGE results of human Fc-tagged protein in Case 1.
Lane 1: Targeted Protein; Lane 2: BSA.
Fig.2. The SDS-PAGE results of His-tagged protein in Case 2.
Lane 1: Targeted Protein; Lane 2: BSA.
Our mammalian cell protein expression service has been developed from our core expertise in the field of gene expression and protein synthesis. Additional yield enhancement through better translation efficiency (more protein per mRNA) improves economics for antibodies and other biopharmaceutical proteins. Please contact us for more information.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.