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Protocol for Biotin-Antibody Conjugation
Disclaimer
This procedure should only be used as a guideline. Please keep in mind that Creative Biolabs cannot guarantee the functionality of client-conjugated antibodies or peptides.
Procedures
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Prepare LCB-NHS (long-chain biotin NHS ester) solution with DMSO (6 mg/ml, 6.5 mM).
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Add 10 µl of LCB-NHS to 1 ml of 2 mg/ml Ab and incubate at RT for 50 min.
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Add 0.5 ml of 1 M Tris.HCl, pH 8.0 to terminate the reaction.
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Dialyze with 1X PBS for 24-48 hours.
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Add 0.1% (w/v) NaN3 if necessary.
Tips
This protocol can be used for any peptide and protein.
Antibody-Biotin Conjugation Service
Creative Biolabs provides professional service for projects involving antibody-biotin conjugation.
Protein and Antibody Biotinylation
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.