Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications. Highlights
Creative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
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HighlightsSolubility is an important physicochemical parameter for drug candidates and is crucial in both drug discovery and development process. Poor solubility is detrimental to absorption after oral administration and may cover compound activity in bioassays in different ways. As such, solubility properties should be ideally identified as soon as possible in the early phase of drug development. With a high reputation in the bio-manufacturing market, Creative Biolabs has introduced high-throughput kinetic and thermodynamic assays in drug discovery to make an estimate of a compound's solubility, which guides our clients the best option to access a potential drug candidate before the clinical stage.
Compound molecules need to be dissolved to undergo pharmacological processes in vivo (e.g. intestinal absorption) and in vitro (e.g. permeation, metabolism, bioassay, and target binding). The solubility of a compound is dependent on its structure and dissolving conditions (e.g. solution components and physical conditions). Structure decides the lipophilicity, ionizability, hydrogen bonding, molecular volume, and crystal energy, which determine solubility. Dissolving conditions are affected by pH, additives, co-solvents, ionic strength, as well as time and temperature. Numerous drug discovery assays are managed under kinetic solubility conditions. In drug discovery, solubility exerts a great influence on bioassays, formulation for in vivo dosing, and intestinal absorption.
Technically, solubility is defined as the concentration of a compound at equilibrium with excess solid (saturated solution) of the most stable crystal structure under specific physical and chemical solution conditions. Low solubility will lead to unpredictable and unreliable results during in vitro testing, thereby increasing the research costs. At the later stage of drug discovery, solubility issues may cause poor bioavailability, undervalued toxicity, and other obstacles, reducing the chances of a drug candidate for success.
Fig.1 Schematic diagram of the recommended workflow for automated high-throughput solubility determination adapted from the SORESOS assay. (Sou, 2018)
The rapid determination of compound solubility is an essential tool for the early drug discovery process, as well as for lead optimization. As a key physical property, it can be measured as either a kinetic or thermodynamic value. At Creative Biolabs, we provide a wide range of methods to accurately determine compound solubility, since good solubility is required not only for obtaining reliable results in early drug discovery but also enabling sufficient concentration of a drug being in circulation to get the optimum exposure. Our precise solubility assays including but not limited to:
The kinetic solubility is the maximum solvability of the fastest precipitating species of a compound. It is often measured by a stock solution of the compound dissolved in an organic solvent as the starting material, typically dimethyl sulfoxide (DMSO). This determination is performed in high throughput with shorter incubation times and final high-throughput analyses using plate readers.
Conventional kinetic solubility assays
Extended kinetic solubility assays
The thermodynamic solubility is the saturation solvability of a compound at the end of the dissolution process, where the dissolved compound is in equilibrium with the undissolved material in excess. This type of solubility depends on dispensing a solid compound into a solvent. It is normally deemed as the true solubility of a compound and is a critical parameter in formulation development.
The solubility indicates the importance of measuring it for drug discovery compounds. Accurate understanding and control of a compound solubility are beneficial to fruitful drug research and development. As an expert in the bio-manufacturability assessment, Creative Biolabs provides a variety of solubility measurement assays according to the special needs of clients’ respective situations, which ensures their productive drug discovery activities, including structure design for optimizing the solubility, determining effects of solubility on drug discovery assays. For more information, please directly contact us or consult our experts online.
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