Creative Biolabs proudly brings out the unique monoclonal shark antibody production service for our customers all over the world. Through our elegant shark immunization and phage display technology, the novel monoclonal shark antibodies can be obtained with high specificity and affinity.
The cartilaginous fishes are known as the oldest phylogenetic group from evolutionary perspective in which possess a “modern” human-type adaptive immune system and immunoglobulins. As one typical species in this family, sharks have been around for over 500 million years. Due to survive in marine environment, large quantities of urea are contained in sharks’ blood to prevent dehydration, while also make their antibodies much more robust than that of other vertebrates. Comparing with the various immunoglobulin isotypes produced by other species, sharks, on the other hand, mainly produce IgM, IgW and IgNAR (immunoglobulin new antigen receptor). IgNAR is a heavy chain homodimer, which consists of five constant domains and one variable region (VNAR) for each heavy chain. Meanwhile, relevant researches also indicated that IgNAR has several disulfide bonds in unusual positions, an extended loop formed in CDR3, a deletion in the FR2-CDR2 region and smaller size than standard antibodies. These differences result in remarkable stability and penetrability of IgNAR and enable the function under extreme conditions. Simultaneously, it is much easier for VNAR to access to clefts, grooves or buried epitopes from the target surface that cannot be reached by larger antibody fragments, and it can also penetrate into dense tissues, such as tumors. In this way, VNAR is also considered a wonderful source for the development of single domain antibodies, which can contribute to various researches and pharmaceutical applications.
Currently, Creative Biolabs offers this unique opportunity for our global clients to obtain novel antibodies directly from immunized sharks. Combining with our well-recognized phage display technology, our scientists are pleased to generate phage display shark antibody libraries from immunized sharks and isolate high specific and affinity monoclonal shark antibodies using biopanning techniques. With this strategy, we can generate single domain shark antibodies for the most specific target. As alternative, Creative Biolabs also provides Premade Shark Antibody Library screening service and the unique Native™ Shark Antibody Discovery Service, which is based on antigen-specific B lymphocytes cytometry technology.
Creative Biolabs is professional in developing high specificity and affinity monoclonal antibodies of various species (e.g. shark, rabbit, chicken, llama, camel, dog, mouse, rat and human). Based on years of experience and advanced technologies, our devoted scientists are confident in tailoring the most appropriate methods to achieve our customers’ objectives.