Broccoli-derived Exosome Research and Application

Broccoli is a highly nutritious vegetable rich in bioactive compounds that contribute to cell protection, reduce the risk of chronic diseases, inhibit cancer development, and enhance immunity. Regular consumption of broccoli can be a part of promoting overall health. Creative Biolabs is a biotechnology company that offers a range of exosome-related biotechnology services and is confident in providing technical support for potential broccoli-derived exosome development.

Multiple Benefits of Broccoli

Antioxidant Action: Broccoli is rich in antioxidant components such as ascorbic acid, carotenoids, vitamin E, and phenolic compounds, which help eliminate free radicals and reduce cell oxidative damage.
Anti-Cancer Effect: Active components in broccoli, such as sulforaphane and glucoraphanin, have been associated with reduced cancer risk. They can regulate intracellular antioxidant enzymes, activate anti-cancer genes, and inhibit the growth and spread of cancer cells.
Cardiovascular Protection: Various components in broccoli, including carotenoids and sulfides, can help improve cardiovascular health by reducing cholesterol levels, protecting blood vessel walls, and reducing the risk of blood clot formation.
Immune Regulation: Active components in broccoli can enhance the body's resistance to infections and diseases by modulating the immune system.
Anti-Inflammatory Effect: Certain components in broccoli have the potential to control inflammatory responses, helping alleviate symptoms of chronic inflammation-related diseases.

Summary of Broccoli-Derived Exosome Research

Broccoli has been extensively studied for its nutritious components and bioactive compounds, particularly its anti-cancer properties. Therefore, researching broccoli-derived exosomes may help uncover these potential health benefits.

Potential Function	Target	Possible Mechanism	Representative Figure
Anti-Colitis	Dendritic cells (DCs)	Broccoli-derived exosomes induced tolerogenic AMPK+ DCs, effectively inhibiting mouse colitis.	Fig.1 Schema for broccoli-derived exosomes mediated induction of tolerogenic AMPK+ DC.¹
Anti-Melanoma	Melanoma cells	Sulforaphane encapsulated in broccoli-derived exosomes reduced melanoma cell proliferation by promoting metabolite generation and reducing cancer molecular markers.	Fig.2 Phase-contrast microscopy images of SK-MEL-28 cells treated with different treatments.²
Anti-Cancer	Human colorectal adenocarcinoma cells	Broccoli-derived exosomes encapsulating astaxanthin significantly inhibited the proliferation of human colorectal adenocarcinoma cells.	Fig.3 Characterization of broccoli-derived exosomes loaded with astaxanthin.³
Anti-Cancer and Antioxidant Effects	Cancer cells	Broccoli-derived exosomes containing antioxidant compounds reduced oxidative stress in various cancer cell lines, impacting cancer cell metabolism.	Fig.4 Efficacy of broccoli-derived exosomes on oxidative stress.⁴
Delivery of Therapeutic miRNA	Human colorectal adenocarcinoma cells	Broccoli-derived exosomes encapsulating therapeutic miRNA promoted the death of human colorectal adenocarcinoma cells compared to free miRNA treatment.	Fig.5 Therapeutic potential of broccoli-derived exosomes as nanocarriers of exogenous miRNAs.⁵
Anti-Cancer	Human pancreatic cancer cells	Exosomes derived from selenium-rich broccoli are loaded with miR167a, which significantly promoted apoptosis in human pancreatic cancer cells by regulating the IRS1/PI3K/AKT pathway.	Fig.6 Selenium biofortification enhanced miR167a expression in broccoli extracellular vesicles inducing apoptosis in human pancreatic cancer cells.⁶

Creative Biolabs leads in the field of plant exosome extraction and development services, providing innovative solutions to support broccoli-derived exosome research. For further information or collaboration, please feel free to contact us for detailed technical support and customized services. These services include:

Plant-Derived Exosome Isolation

Plant-Derived Exosome Identification

High-Throughput Screening Analysis (Proteins, RNA, Lipids and Metabolites)

Target Analysis

In Vitro Functional Discovery of Plant-Derived Exosomes

In Vivo Functional Discovery of Plant-Derived Exosomes

Large-Scale Production of Plant-Derived Exosomes

References

Deng, Z.; Rong, Y.; et al. Broccoli-derived nanoparticle inhibits mouse colitis by activating dendritic cell AMP-activated protein kinase. Molecular Therapy. 2017. 25(7):1641-1654.
Yepes-Molina, L.; Carvajal, M. Nanoencapsulation of sulforaphane in broccoli membrane vesicles and their in vitro antiproliferative activity. Pharmaceutical Biology. 2021. 59(1):1490-1504.
Li, C.; Song, Q.; et al. Preparation, characterization, and in vitro anticancer activity evaluation of broccoli-derived extracellular vesicle-coated astaxanthin nanoparticles. Molecules. 2022. 27(12).
Hossain, MN.; De Leo, V.; et al. Characterization of anti-proliferative and anti-oxidant effects of nano-sized vesicles from Brassica oleracea L. (Broccoli). Scientific Reports. 2022. 12(1):14362.
Del Pozo-Acebo, L.; López de Las Hazas, MC.; et al. Therapeutic potential of broccoli-derived extracellular vesicles as nanocarriers of exogenous miRNAs. Pharmacological Research. 2022. 185:106472.
Wang, X.; Wu, B.; et al. Selenium biofortification enhanced miR167a expression in broccoli extracellular vesicles inducing apoptosis in human pancreatic cancer cells by targeting IRS1. International Journal of Nanomedicine. 2023. 18:2431-2446.