Tomato-derived Exosome Research and Application

Multiple researchers have already extracted exosomes from tomato juice to explore their potential benefits. This is because tomato-derived exosomes may carry bioactive substances that can be used for health and medical research. Combined with their natural origin from food, they hold promising applications. Creative Biolabs supports the extraction and functional development of tomato-derived exosomes.

Tomatoes are Nutritionally Rich and Offer Various Health Benefits

Anti-Aging: Tomatoes boast a wealth of lycopene, a potent antioxidant renowned for safeguarding the skin against oxidative harm induced by free radicals, ultraviolet (UV) radiation, and extrinsic influences. Lycopene actively fosters cellular proliferation and rejuvenation, thereby acting as a bulwark against the onset of skin senescence, the emergence of hyperpigmentation, and the formation of fine lines and creases.
Cardiovascular Health: Lycopene in tomatoes protects low-density lipoprotein from oxidative damage, reducing the risk of cardiovascular diseases, especially in individuals with high cholesterol levels. Tomatoes are also rich in potassium, helping maintain normal blood pressure.
Prostate Health: Consuming tomatoes and lycopene is associated with reduced risk of prostate diseases, including prostate cancer. Lycopene contributes to maintaining and repairing prostate health.
Anti-Cancer: Tomato extracts contain compounds that inhibit tumor growth, reducing the risk of breast cancer, prostate cancer, and other types of cancer.
Blood Pressure Regulation: Tomatoes' potassium content helps maintain a balance of potassium and sodium, reducing the risk of high blood pressure.
Skin Health: Tomato's lycopene and other antioxidants protect the skin from UV damage, maintaining skin health.

Research Findings on Tomato-Derived Exosomes

Article	Representative Figures
Research Methods: Extraction of tomato-derived exosomes through ultracentrifugation. Assessment of cytotoxicity and anti-inflammatory activity on lipopolysaccharide-stimulated human monocytic cells. Proteomic analysis and bioinformatics. Loading of curcumin into tomato-derived exosomes using different methods. Research Results: Tomato-derived exosomes significantly inhibit the expression of the inflammatory cytokine IL-1β induced by LPS. These exosomes contain proteins associated with various cellular processes, including inflammation. Loading curcumin via sonication enhances the anti-inflammatory activity of tomato-derived exosomes.	Fig.1 Schematic representation of the process to prepare curcumin loaded tomato-derived exosomes.1
Research Methods: Preparation of exosome-like vesicles using an aqueous two-phase system to isolate them from tomato and lemon. Cell experiments to assess toxicity and their impact on the chondrogenic differentiation of adipose-derived stem cells. Research Results: Tomato-derived exosome-like vesicles improve the survival of adipose-derived stem cells without toxicity. These vesicles promote chondrocyte formation, suggesting their potential for cartilage regeneration and osteoarthritis treatment.	Fig.2 Tomato-derived exosomes promote the formation of chondrocytes.²
Research Methods: Extraction of extracellular vesicles from tomato root exudates via ultracentrifugation. Proteomic analysis of these vesicles. Co-cultivation of tomato-cultivated plant pathogens with tomato root-derived extracellular vesicles. Research Results: Proteomic analysis revealed the presence of various proteins related to plant-microbe interactions in these vesicles. Tomato root-derived extracellular vesicles significantly inhibit spore germination and germination tube development of plant pathogens, suggesting their role in the plant immune system.	Fig.3 Experimental design used for collection and characterization of exosomes released by tomato roots.³

Article

Representative Figures

Research Methods:

Extraction of tomato-derived exosomes through ultracentrifugation.
Assessment of cytotoxicity and anti-inflammatory activity on lipopolysaccharide-stimulated human monocytic cells.
Proteomic analysis and bioinformatics.
Loading of curcumin into tomato-derived exosomes using different methods.

Research Results:

Tomato-derived exosomes significantly inhibit the expression of the inflammatory cytokine IL-1β induced by LPS.
These exosomes contain proteins associated with various cellular processes, including inflammation.
Loading curcumin via sonication enhances the anti-inflammatory activity of tomato-derived exosomes.

Schematic representation of the process to prepare curcumin loaded tomato-derived exosomes. (Mammadova, et al., 2023)

Fig.1 Schematic representation of the process to prepare curcumin loaded tomato-derived exosomes.1

Research Methods:

Preparation of exosome-like vesicles using an aqueous two-phase system to isolate them from tomato and lemon.
Cell experiments to assess toxicity and their impact on the chondrogenic differentiation of adipose-derived stem cells.

Research Results:

Tomato-derived exosome-like vesicles improve the survival of adipose-derived stem cells without toxicity.
These vesicles promote chondrocyte formation, suggesting their potential for cartilage regeneration and osteoarthritis treatment.

Tomato-derived exosomes promote the formation of chondrocytes. (Yıldırım, et al., 2023)

Fig.2 Tomato-derived exosomes promote the formation of chondrocytes.²

Research Methods:

Extraction of extracellular vesicles from tomato root exudates via ultracentrifugation.
Proteomic analysis of these vesicles.
Co-cultivation of tomato-cultivated plant pathogens with tomato root-derived extracellular vesicles.

Research Results:

Proteomic analysis revealed the presence of various proteins related to plant-microbe interactions in these vesicles.
Tomato root-derived extracellular vesicles significantly inhibit spore germination and germination tube development of plant pathogens, suggesting their role in the plant immune system.

Experimental design used for collection and characterization of exosomes released by tomato roots. (De Palma, et al., 2020)

Fig.3 Experimental design used for collection and characterization of exosomes released by tomato roots.³

In conclusion, tomato-derived exosomes hold significant potential for applications not only in medical and biomedical research but also in improving plant health and environmental management. Creative Biolabs is committed to providing high-quality plant exosome development services. If you have any needs or questions, please feel free to contact us.

Plant-Derived Exosome Isolation

Plant-Derived Exosome Identification

High-Throughput Screening Analysis (Proteins, RNA, Lipids and Metabolites)

Target Analysis

In Vitro Functional Discovery of Plant-Derived Exosomes

In Vivo Functional Discovery of Plant-Derived Exosomes

Large-Scale Production of Plant-Derived Exosomes

References

Mammadova, R.; Maggio, S.; et al. Protein biocargo and anti-inflammatory effect of tomato fruit-derived nanovesicles separated by density gradient ultracentrifugation and loaded with curcumin. Pharmaceutics. 2023. 19;15(2).
Yıldırım, M.; Ünsal, N.; et al. Effect of solanum lycopersicum and citrus limon-derived exosome-like vesicles on chondrogenic differentiation of adipose-derived stem cells. Applied Biochemistry and Biotechnology. 2023.
De Palma, M.; Ambrosone, A.; et al. Plant roots release small extracellular vesicles with antifungal activity. Plants (Basel). 2020. 9(12).

Plant Exosome Isolation