Butterbur-derived Exosome Research and Application

Butterbur, scientifically known as Petasites japonicus, is an herbaceous plant with a long history in traditional Chinese herbal medicine and folk medicine, being used for various medicinal and culinary purposes. Butterbur has also been the subject of modern medical research due to its potential biological activities such as anti-cancer, anti-inflammatory, and antioxidant properties. By studying exosomes derived from butterbur, we can gain deeper insights into its bioactive components and potential medicinal uses, thereby developing new drugs or therapeutic approaches. Creative Biolabs presents research and potential applications of butterbur-derived exosomes.

Pharmacological Activities of Butterbur

Blood Pressure Regulation: Alcoholic and ether extracts of butterbur significantly elevate blood pressure in cats. Ketones found in butterbur also exhibit dose-dependent blood pressure elevation.
Neuroprotection: Butterbur can alleviate neurogenic damage caused by arachidonic acid and nitric oxide, as well as neurotoxicity associated with neurodegenerative diseases.
Digestive Promotion: Butterbur extracts have inhibitory effects on diarrhea and gastric ulcers and can stimulate bile secretion.
Antioxidant: Butterbur demonstrates pharmacological effects in protecting the body from oxidative damage at the cellular level. Some compounds can neutralize reactive oxygen species, reducing cellular oxidative damage.
Anti-Inflammatory: Butterbur can reduce the generation and secretion of various inflammatory factors.
Respiratory Comfort: Butterbur exhibits antitussive, bronchodilatory, and expectorant activities in some animal models, which may aid in the treatment of respiratory diseases.
Anti-Cancer Potential: The examination of individual monomeric constituents within butterbur has yielded compelling evidence of their ability to impede the proliferation of cancer cells.

Research on Butterbur-Derived Exosomes

It should be noted that while butterbur displays these pharmacological activities, the specific mechanisms of action require further in-depth research for clarification. Currently, there is limited information available regarding butterbur-derived exosomes. However, this suggests that research on butterbur-derived exosomes may still be in its early stages, and researchers and companies may consider conducting more experiments and studies to better understand the biological effects of butterbur-derived exosomes.

Title: Immunostimulatory Potential of Extracellular Vesicles Isolated from an Edible Plant, Petasites japonicus, via the Induction of Murine Dendritic Cell Maturation.

Research Methods:

Extraction and characterization of butterbur-derived exosomes from leaves using a combination of filtration and differential centrifugation.
Evaluation of the immunomodulatory effects of butterbur-derived exosomes on dendritic cells (DCs) by assessing changes in the expression of DC surface molecules, production of Th1-polarizing cytokines, antigen presentation capability, and antigen uptake capability.
Investigation of the mechanism by which butterbur-derived exosomes induce DC maturation through the activation and phosphorylation of intracellular MAPK and NF-κB signaling pathways via Western blotting.
Assessment of the impact of butterbur-derived exosomes on T cell proliferation and differentiation, as well as their ability to induce Th1-type T cells and cytotoxic CD8+ T cells through mixed lymphocyte reactions (MLR) and cytokine analysis.
Evaluation of the anti-tumor effects of orally administered butterbur-derived exosomes on immune cells in a tumor-bearing mouse model.

Research Findings:

Butterbur-derived exosomes stimulate DC maturation, heightened production of Th1-polarizing cytokines, and enhanced antigen presentation capability.
This DC maturation process hinges on the activation and phosphorylation of the MAPK and NF-κB signaling pathways.
DCs exposed to butterbur-derived exosomes robustly facilitate the proliferation and differentiation of naive T cells into Th1 cells and cytotoxic CD8+ T cells.

Conclusion: Butterbur-derived exosomes possess immunostimulatory potential, inducing the maturation and activation of dendritic cells and enhancing the immune response of T cells. These results provide a theoretical basis for the use of butterbur-derived exosomes as a potential immunotherapeutic and drug delivery nanoplatform.

Fig.1 DCs treated with PJ-EVs initiate T cell proliferation and Th1 response.¹

Creative Biolabs' professional team has extensive experience and expertise in the field of exosome research and can provide high-level solutions for plant-derived exosome research. If you or your team are interested in conducting research on butterbur-derived exosomes, please feel free to contact us. Our expertise and comprehensive services will provide strong support for your research, helping you achieve more scientific advancements.

Plant-Derived Exosome Isolation

Plant-Derived Exosome Identification

High-Throughput Screening Analysis (Proteins, RNA, Lipids and Metabolites)

Target Analysis

In Vitro Functional Discovery of Plant-Derived Exosomes

In Vivo Functional Discovery of Plant-Derived Exosomes

Large-Scale Production of Plant-Derived Exosomes

Reference

Han, JM.; Song, HY.; et al. Immunostimulatory potential of extracellular vesicles isolated from an edible plant, petasites japonicus, via the induction of murine dendritic cell maturation. International Journal of Molecular Sciences. 2021, 22(19)

Plant Exosome Isolation