Extracellular Vesicle Cytokine Profiling Service
Overview Services Features FAQs
Overview
The Cytokines in Extracellular Vesicles
Extracellular vesicles (EVs) are nanoscale lipid vesicles secreted by cells into the extracellular matrix. In addition to containing different kinds of biomolecules such as miRNAs, mRNAs, proteins, and lipids, EVs also carry mother cell-derived cytokines. Cytokines are a class of small molecular proteins with multiple functions, mainly involved in immune cell differentiation, mediating immune response and inflammatory response, stimulating hematopoietic function and tissue repair, etc. At present, a large number of studies have only focused on the changes of cytokines in the free state in body fluids, and have not recognized the changes of cytokine in EV in body fluids. This is incomplete for understanding the cytokine profile in body fluids. Because of the stable existence of EVs in the body fluid circulation, the cytokines of EV can be regarded as a good marker. Screening the differential cytokines in EV in disease states can not only find specific disease biomarkers but also provide a new opportunity for analyzing the mechanism of disease occurrence and development.
Creative Biolabs can provide isolation and purification services for EV, characterization and quantification services for EV, EV protein extraction, and cytokine profiling services of different scales to help customers analyze cytokines in EV.
Services
Extracellular Vesicle Cytokine Detection Service
The cytokine detection service we provide is based on enzyme-linked immunosorbent assay (ELISA), which is especially suitable for the quantitative detection of a specific cytokine in a large number of EV samples. The basic principle is to combine the cytokines in EV as antigens with the corresponding enzyme-labeled antibodies. Due to the high frequency of enzyme catalysis, the final color reaction can amplify the binding reaction of cytokines and antibodies, thereby realizing the quantitative detection of cytokines in EV.
Extracellular Vesicle Cytokine Profile Detection Service
The cytokine profile detection service we provide is based on the cytokine liquid-phase suspension chip technology, which is especially suitable for the quantitative detection of multiple specific cytokines in large batches of EV samples. The basic principle is to couple highly specific antibodies to different fluorescently labeled magnetic beads, mix these antibody-labeled magnetic beads and suspend them in a 96-well microwell plate. Further, by adding a biotin-labeled high-affinity detection antibody, the target cytokines captured by the magnetic beads are simultaneously recognized. During detection, one laser beam recognizes the capture antibody labeled with magnetic beads to know which cytokines can be detected by the magnetic beads, and the other laser beam is irradiated for signal amplification detection to realize the quantification of cytokines in EV. We can provide a variety of cytokine liquid-phase suspension chips for customers to choose from according to their actual needs.
Features
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Comprehensive Cytokine Detection
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High Sensitivity and Accuracy
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Expert Guidance and Support
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Valuable Insights and Results
Creative Biolabs has been committed to mining the biological information in EV and can provide cytokine detection services for researchers. Whether it is the early screening of a large number of candidate cytokines and biomarkers or the large-scale verification of specific cytokines and biomarkers, you can find suitable technology platforms and services here to meet your research needs. If you are interested in EV cytokines, please contact us and make a list of cytokines you want to detect. Our professional scientific team will formulate the most suitable cytokine detection plan for you for the first time.
FAQs
Q: How much sample volume is needed for cytokine analysis in extracellular vesicles?
A: Different technical methods for analyzing exosome cytokines require different sample volumes. For ELISA, we typically need at least 20 µL of exosomes per target per sample, while cytokine arrays may require a slightly larger volume, around 100 µL of exosomes per sample. You can submit your project description and any other questions on the right side of the page. We will contact you as soon as possible to determine the minimum sample volume required and other sample requirements (such as concentration, aliquoting, and transportation requirements).
Q: How many cytokines can be analyzed simultaneously in extracellular vesicle cytokine analysis?
A: Multiple cytokines can be analyzed simultaneously in a single sample. The number of cytokines detected depends on the species and the specific panel selected. Based on our communication, we will assist you in selecting the cytokine panel that best suits your project.
Q: What quality controls do you use to ensure the reliability of cytokine measurements?
A: We implement strict quality control measures at every step of the analysis, including quantitative standard curves and setting up replicates to ensure the accuracy and consistency of the results.
Q: How do you handle potential interference or background noise in cytokine analysis?
A: We perform background subtraction to minimize potential interference or background noise.
For Research Use Only. Cannot be used by patients.
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