ExoTEST is a sandwich ELISA assay for quantification and characterization of exosomes in cell culture supernatant and biological fluids. The non-invasive protein biomarkers of exosome can be used for detection and monitoring of a number of pathological conditions. Creative Biolabs has successfully launched an innovative exosome characterization platform based on ExoTEST.

Enzyme-linked Immunosorbent Assay (ELISA)

Enzyme-linked immunosorbent assay (ELISA) is both widely used as diagnostic tools in medicine and as quality control measures in various industries;it is also used as analytical tools in biomedical research for the detection and quantification of specific antigens or antibodies in a given sample. ELISA uses the basic immunology concept of an antigen binding to its specific antibody, which allows detection of very small quantities of antigens such as proteins, peptides, hormones, or antibody in a fluid sample. ELISA utilizes enzyme-labeled antigens and antibodies to detect the biological molecules, the most commonly used enzymes being alkaline phosphatase and glucose oxidase.

Exosome Characterization via ExoTEST

ExoTEST specifically capture exosomes by exosome specific protein antibodies from different biological samples, including cell culture supernatant and biological fluids. The quantification and characterization of exosomes proteins are followed by the use of appropriate detection antibodies to detect the exosomes associated antigens, which can be generic or cell-specific exosomes. Exosome can perform different biological functions due to its different proteins. For example, exosome secreted by tumor cells can mediate the proliferation and immune escape of angiogenic tumor cells. Dendritic cell-derived exosomes can elicit an effective anti-tumor immune response. Thus, the non-invasive protein biomarkers of exosome can be used for detection and monitoring of a number of pathological conditions.

Fig.1 Schematic workflow depicting sequential molecular binding events of the sandwich ELISA.

Features of Our Services

• No initial exosome purification required
• Feasible for multiple markers analyze
• High throughout
• Skillful scientific team
• Best after-sale service

With the help of our well-established technologies and experienced scientists, Creative Biolabs provides customized ExoTEST service for customers all over the world. We have abundant antibody resources to meet requirements of different sources exosome. Send us fresh cell culture supernatant and biological fluids for detection of target exosome, a complete data reports including experimental procedures, methods, reagents, instruments, standard curves and data analysis will send back to you. We provide very flexible options for each specific case. We are happy to make it accessible to all kinds of research and industrial customers. Please don’t hesitate to contact us for more information.

• Exosome Characterization via Western Blotting
• Exosome Characterization via FACS