Exosome characterization is difficult due to their size and lack of discrete positive populations. Empowered by leading technologies as well as years of experience in this field, Creative Biolabs has successfully launched an innovative exosome characterization platform based on fluorescence-activated cell sorting (FACS) technology.

Exosome Characterization

Exosomes are small, membrane-derived vesicles found in bodily fluids that are involved in cell-cell communication and help regulate a diverse range of biological processes. Through expression of various surface markers and/or direct transfer of biological material, exosomes are able to alter the function of recipient cells to play either activating or suppressing roles in intercellular communication.

In order to analyze exosomes, researchers have utilized various methods, including immunoblotting, ELISA and bead capture-based flow cytometry, along with newer technologies such as nano-plasmonic sensor detection. These approaches largely provide bulk measurements and are unable to precisely sort distinct subsets of vesicles for further characterization. High-resolution imaging modalities such as cryo-EM and atomic force microscopy provide exceptional resolution of exosomes; however, these modalities have slow throughputs and are capable of analyzing only a few parameters simultaneously. Moreover, these specialized imaging techniques do not allow for sorting and purification of exosomes for subsequent analysis. Using an optimally configured FACS flow cytometer, we provide service to analyze and sort exosomes based solely on the presence of endogenous membrane constituents.

Exosome Characterization via FACS

Exosome Characterization employs the FACS analysis technology to provide fast and accurate quantitative protein characterization and exosome sorting. Our highly experienced expert team executes quality management, following every procedure to ensure confident and unbiased results. The general workflow for FACS analysis process is listed below.

Features and Advantages of Our Services

• High throughput
• Low background fluorescence
• High exosome purity
• Skillful scientific team
• Best after-sale service

With the help of our well-established technologies and experienced scientists, Creative Biolabs is capable of characterizing and sorting exosome via FACS. The techniques developed by us assist with eliminating the antibody aggregates commonly found in commercial preparations, increasing signal–to-noise ratio, and setting gates in a rational fashion that minimizes detection of background fluorescence. In addition, we have abundant antibody resources to meet requirements of different sources exosome. We provide very flexible options for each specific case. When you choose us, you can get all related data of your exosomes and without needing to invest in your own NTA instrument and optimize conditions. Send us your sample or prepared exosomes, choose analysis or sorting, and we will send back a report with the particle protein analysis data or purified exosome. Please don’t hesitate to contact us for more information.

• Exosome Characterization via Western Blotting
• Exosome Characterization via ExoTEST (ELISA)