Exosome Characterization via Western Blotting

Western Blot is an experimental method commonly used in molecular biology, biochemistry and immune genetics. Based on years of experience in exosome characterization, Creative Biolabs has successfully launched an innovative exosome characterization platform based on western blotting.

Western Blotting

Western blotting (also known as immunoblotting) is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture. Originally conceived by Towbin et al., the technique enables indirect detection of proteins immobilized on a nitrocellulose or PVDF membrane. Given the relatively low cost, sensitivity, and flexibility of western blotting, thousands of research labs around the world have employed its use to measure protein levels. Especially with the recent availability of commercially produced antibodies, western blotting is becoming a popular technique in the life sciences. As a convenient and reliable research tool, WB can be used for subsequent analysis of protein-protein, protein-DNA and protein-RNA interactions through semi-quantitative detection of target protein expression through antigen-antibody reaction, and will play an important role in proteome era together with technologies such as spectrum and protein chip.

Fig.1 Identification of a membrane form of TNF-α on the RASF exosomes. Exosomes were analyzed by Western blotting with a monoclonal anti-TNF-α Ab (A) and the molecular mass of exosomal TNF-α is determined(B). (Zhang, 2006)

Workflow of Exosome Characterization via Western Blotting

As there are no exosome specific markers, proteins that are enriched in exosomes, from all different cellular origins, are commonly used for exosome detection. Thus, the markers for exosome detection may vary depending on its parent cells. The general workflow for Western Blotting analysis process is listed below. Notablly, we have special protein isolation method before the Western blot, and will help you choose the suitable antibodies. Our highly experienced expert team executes quality management, following every procedure to ensure confident and unbiased results.

Fig.2 Western Blotting Workflow. (Jeong, 2012)

Features and Advantages of Our Services

Special methods designed for exosome protein isolation
High quality pictures with low background and bright bands
Abundant antibody resources
Skillful scientific team
Best after-sale service

The Western Blot method is very mature, the most lab has equipment to finish it. However, getting good results requires a lot of time and effort for optimization. With the help of our well-established technologies and experienced scientists, Creative Biolabs has special skills in exosome protein isolation, transprinting and antibody dilution. We customize the experiment plan for customer samples. We have abundant antibody resources to meet requirements of different sources exosome, and provide flexible options for each specific case. We are happy to make it accessible to all kinds of research and industrial customers. Please don’t hesitate to contact us for more information.

References:

Jeong, D.E.; et al. Western Blot Analysis of C. elegans Proteins. Methods Mol Biol. 2018, 1742:213-225.
Zhang, H.G, et al. A membrane form of TNF-α presented by exosomes delays T cell activation-induced cell death. The Journal of Immunology. 2006, 176(12): 7385-7393.

Plant Exosome Isolation