Stability Evaluation Service for Exosome Product
Exosomes are vesicles secreted by cells with both inheritance and transmission functions. Coupled with the properties that can be engineered, exosomes have been shown to have potential applications in fields such as medicine, biotechnology, and drug development. Large-scale production of exosomes can meet the needs of academia and industry for exosome products, laying a solid foundation for transforming exosome research into applications. In the large-scale production process, the stability evaluation of exosome products is indispensable. In order to meet the needs of customers, Creative Biolabs has specially deployed the stability evaluation business of exosome products.
Fig. 1 Extracellular vesicle product characterization. (Porzionato, 2019)
Why Need To Evaluate the Stability of Exosome Products?
There are differences in the stability of exosomes under different preparation methods and storage conditions. Improper handling may damage and even degrade exosomes, resulting in changes in their composition, activity, and function, thereby affecting their application effects. Therefore, the evaluation of exosome product stability helps in the following ways.
Stability Evaluation Service of Exosome Products at Creative Biolabs
Using rich experience and advanced technology platforms, Creative Biolabs will provide the best exosome product stability evaluation service.
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Stability Evaluation Angle
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Evaluation Reason
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Test Methods
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Evaluation method
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Preparation method
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Although some preparation methods ensure the purity of exosomes, they have a certain degree of damage to exosomes. Currently, commonly used exosome isolation methods include ultracentrifugation, ultrafiltration, affinity chromatography, size-exclusion chromatography, and Precipitation. The quality of exosomes obtained by these methods is also different.
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Quality comparison of exosomes from different preparation methods
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Storage temperature
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Since frequent freezing/thawing processes may cause damage to exosomes during cryopreservation, it is necessary to evaluate the stability of exosomes after 1-2 freezing/thawing cycles.
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Freeze/thaw test
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In general, exosome products can be cryogenic or freezing. Therefore, it is necessary to evaluate the stability of cryogenic or frozen exosomes.
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Storage temperature comparison
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Storage time
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As biologically active vesicles, exosomes may be degraded and lose their effectiveness after being placed for a long time. Therefore, it is necessary to compare the differences in parameters between fresh exosomes and exosome products after a period of storage.
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Comparison at different storage time points
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Stock buffer composition
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Adding some protective agents to the storage buffer can improve the stability of exosomes. Therefore, it is necessary to compare the protective effects of different buffer formulations on exosomes.
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Stock buffer composition comparison
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Other feasible evaluation angles
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Concrete analysis of specific situations
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Through these refined analyses and evaluations, we will ensure that the quality and stability of exosome products are maximized, and help customers ensure the reliability of exosome products. If you need to evaluate the stability of your exosome product, please contact us and tell us the specific information about the product and testing requirements to get a complete quotation.
Reference
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Porzionato, A.; Zaramella, P.; et al. Intratracheal administration of clinical-grade mesenchymal stem cell-derived extracellular vesicles reduces lung injury in a rat model of bronchopulmonary dysplasia. American Journal of Physiology. Lung Cellular and Molecular Physiology. 2019. 316(1):L6-L19.
For Research Use Only. Cannot be used by patients.
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