Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsImmortality of the hybridoma cell line is a property of myeloma cells which are fused to antibody-specific B cells. If your hybridoma no longer grows well, re-fusing a hybridoma to new myeloma cells can help. Scientists at Creative Biolabs have extensive experience in hybridoma generating. After years of unremitting efforts, we offer hybridoma re-fusing service for global clients. Creative Biolabs will be your strong support on your way of hybridoma rescue.
The somatic fusion of B lymphocytes of the spleen with immortal myeloma cells gives rise to a hybridoma cell line that can be perpetually propagated to produce clonally identical antibodies. These hybridoma cells inherit the indefinite growth properties of myeloma cells and the antibody secretion capabilities of B-lymphocytes. Monoclonal antibodies (mAbs) produced by a unique hybridoma cell line are homogeneous and recognize a single epitope of an antigen. Cytogenetic studies have confirmed that upon most occasions, the chromosome from one parent may be lost during the proliferation of a hybridoma, re-fusion can be an appropriate method to remedy this situation by increasing the corresponding parental chromosome complement. Sometimes, hybridomas made by fusing myeloma cells with spleen cells from a specific immunized animal fails to maintain antibody secretion in culture. However, when one or more of these hybridomas are selected and then re-fused with immunized spleen cells, several lines that stably secrete antibodies will be obtained.
In the whole process of hybridoma preparation, selection of the optimal myeloma cells is especially critical for achieving the desired hybridoma. Creative Biolabs will re-fuse your hybridoma to two different myeloma cell lines and establish new monoclonal cell lines for you that are selected for optimal growth as well as antibody production. Generally, 1~2 mg target protein is required to test parental re-fusion cultures and subsequent subclones. Besides, collected supernatant samples will be sent to you for testing.
If you are looking for a hybridoma re-fusing service right now, please contact us for more information and a detailed quote.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
