Hybridoma Rescue Service

Many hybridoma researchers have lost an important hybridoma cell line because of instability, mechanical breakdown of a freezer, or contamination. Hybridoma rescue has become a great challenge worldwide. Creative Biolabs possesses diverse flexible, interactive, and cost-effective hybridoma development systems. We now have the capability and technology platforms to offer you diverse hybridoma rescue services.

Hybridoma Rescue

Reinitiating a hybridoma culture from a frozen stock occasionally results in only a small number of viable hybridoma cells. Typically, this is caused by low viability of the hybridoma cells at the time of freezing, poor freezing technique, or improper maintenance of frozen cell stocks. It can be extremely difficult to propagate the few viable cells that survive. Using proprietary technology/medium developed by us, Creative Biolabs has been successful in rescuing hybridomas where only a few cells were viable. Cells are expanded and vials frozen back. Creative Biolabs provides the following rescue services:

Hybridoma Suspension Domestication Service

Cell lines from different sources, and different clones of the same cell line, may have highly specific nutritional requirements and may therefore prefer one medium over another. The use of serum-free medium is now common practice. Protein-free defined media guarantee easy purification and beneficial stability for tracing the antibody product. Our innovative serum-free medium formulation and cell culture process scale-up expertise allow us to provide cost-effective services for hybridoma suspension domestication.

Hybridoma Re-cloning Service

Have you observed that the level of antibodies produced by hybridomas has steadily decreased over time? This could be caused by mainly two reasons. Some cells in the population have become non-producers. Since non-producer cells have a growth advantage over cells that actively produce antibodies, the proportion of non-producer cells will increase as the number of passages increases. On the other hand, the cell line is not monoclonal. In many cases, this is difficult to determine, but if you think this is a possibility, you can isolate the correct clone from the cell stock by further subcloning

Hybridoma Enrichment Service

Multiple rounds of FACS enrichment will be carried out and the top antibody-producing cells are expanded and frozen.

Hybridoma Cell Line Optimization Service

Our hybridoma optimization services can establish new clones that are selected for optimal growth and maximum antibody productivity.

Hybridoma Re-fusion Service

Immortality of hybridoma cell lines is a property of myeloma cells that are fused to antibody-specific B cells. If your hybridoma no longer grows well, that might be a symptom of instability, and re-fusing a hybridoma to new myeloma cells can help. scientists at Creative Biolabs will re-fuse hybridomas to different myeloma cell lines and establish new monoclonal cell lines according to established protocols.

Creative Biolabs specializes in high-quality monoclonal antibodies, particularly those which are difficult to produce. Our two main foci are perfect screening methodologies and excellent hybridoma rescue services, producing high-quality hybridoma cell lines. We also offer multiple additional services for your special needs. If you are interested in our services, please feel free to contact us for more information.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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