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NanoScaffold Development for Target-Specific Cell Penetration

Creative Biolabs has accumulated extensive experience in providing custom scaffold development services. With our cutting-edge proprietary technologies, now we can obtain the target-specific intracellular proteins based on the novel NanoScaffold, CpNS. We are pleased to use our proprietary Hi-Affi™ platform to generate the CpNS libraries with 100% precise mutation and over 1010 diversity.

Introduction of CpNS

With the properties of flexible size, versatile functions, and complex structure, proteins can always recognize and modulate disease-related macromolecules, so that they present great potential as small-molecule reagents. However, most proteins cannot cross the lipid bilayer membrane of mammalian cells, which largely limit the biological therapeutic and diagnostic against intracellular targets. Some studies have shown that cationic resurfacing of proteins can endow cell penetration. The magnitude of the net theoretical charge may influence the characterizations of proteins, which include aggregation, stability, solubility, and also the ability to penetrate the target cells. The problem is many proteins are not amenable to such extensive mutagenesis. Now Creative Biolabs can generate a novel NanoScaffold, known as CpNS, to obtain the cell-penetrating binders against intracellular targets.

Cell pattern diagram

Construction of CpNS Library

The CpNS is composed of a modified framework region with an exposed polycationic surface and three loops available for mutation. According to a variety of modifications, we have got several stable framework available for CpNS design. Based on our advanced nucleotide doping techniques (NNK/NNS, and Trimer Codon strategy), the CpNS library can be constructed through properly design and well control. After a series of screening strategies, the desired cell-penetrating binders can be obtained. In addition, the antigen binding region of existing ligand has a potential possibility to integrated with CpNS scaffold to generate new binders with cell-penetrating ability. It is worth mentioning that reporter proteins can also be fused to CpNS for biological purposes and functions.

Attractive Features of CpNS


Creative Biolabs is one of the well-recognized experts who are professional in applying advanced phage display technologies for a broad range of scaffold protein research. Based on our extensive experience and advanced platforms, we are confident in filling the gaps in the scaffold field. If you are interested in our Fynomers library construction service, please do not hesitate to contact us for more details.


All listed customized services & products are for research use only, not intended for pharmaceutical, diagnostic, therapeutic or any in vivo human use.

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