Sequencing-based Typing (PCR-SBT) Technology

Creative Biolabs provides customers with HLA typing services through polymerase chain reaction-sequencing based typing (PCR-SBT) technology. Our advanced SBT technology introduces dye-labeled primers and automatic fluorescence sequencing. The number of allele sequences in the sequencing template is limited by group-specific amplification, which avoids the interference to the detection results by DNA of the two haplotypes. It is one of the most accurate methods in the HLA typing field.

Introduction to HLA Tissue Typing

HLA is one of the most polymorphic genetic structures in the human genetic system and is composed of a series of closely related genes. The HLA gene is very important in the fields of medicine, anthropology, and forensics. With the development of molecular biology technology, the technical methods for HLA typing are becoming more and more perfect. Currently, PCR-SSP, PCR-SSOP, and PCR-SBT are the most widely used HLA typing methods allow a medium- or high-resolution throughput. The accuracy of these HLA typing methods is critical in medical applications, and in the transplantation of organs and tissues, if there is no correct pairing, it will lead to dangerous rejection. In the past few years, scientists have conducted several comparative studies between serology and DNA technology for HLA typing. And DNA technology is currently more reliable than serological techniques. However, DNA technology is not flawless, their accuracy depends on sensitive procedures conducted at specific temperatures, good DNA quality, adequate reagents, and also correct interpretation of the results.

PCR-SBT for HLA Tissue Typing

One disadvantage of PCR-SSO or RFLP is that although they are capable of detecting single-base differences in DNA sequences between two alleles, they are less likely to detect new undefined alleles unless the variation happens to be at the specific site detected by the probe or the enzyme used for restriction. PCR-SBT was originally developed as a method of manual sequencing, did not require any additional typing information, e.g. serology, and was later applied to Class I alleles. Its superiority is the introduction of dye-labeled primers and fluorescent automated sequencing. This has greatly improved its accuracy. PCR-SBT is the only technique that directly detects the nucleotide sequence of an allele, so it can be accurately assigned. It requires very expensive equipment and complex laboratory methods to provide clear, high-resolution HLA typing results.

Creative Biolabs' newly developed PCR-SBT typing system can be used for high-resolution typing of HLA-A, HLA-B, and HLA-DRB genes. We use terminator sequencing reagents to minimize cost, increase efficiency, and complete the system by providing automatic allele calling and editing.

If you have any questions about our vector design service, you can contact us by email or send us an inquiry to find a complete solution.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

	USA: Europe: Germany:
	Call us at: USA: UK: Germany:
	Fax:
	Email: