Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsWith over a decade of experience in phage display technology, Creative Biolabs can provide a series of antibody or peptide libraries that are available for licensing or direct screening. These ready-to-use libraries are invaluable resources for isolating target-specific binders for various research, diagnostic or therapeutic applications. Highlights
Creative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsGuided selection provides a powerful tool for humanization of the preexisting nonhuman antibodies. Equipped with world-leading technology platforms and professional scientific staffs in phage display, Creative Biolabs has developed an advanced Phage Display Platform to provide a full range of innovative integrated services that are based on phage display technology. Based on this advanced platform, our scientists have developed a sequential guided selection method for the humanization of non-human antibodies. We are pleased to meet every unique requirement and provide the most suitable solution for our client’s satisfaction.
The Background of Guided Selection
Rodent monoclonal antibodies (mAbs) have limitations as therapeutic agents due to their immunogenicity and insufficient activation of human effector functions. This has driven diverse humanization approaches including initial chimeric antibody approach, humanization by CDR grafting, humanization by resurfacing, and deimmunization by the removal of the T-cell epitope. However, the resulting humanized antibodies still have potential to provoke an immune response in humans. As an alternative humanization method, guided selection has been successfully used since 1994. Its value was proved by HUMIRA, the world’s first human antibody approved. The method is based on chain shuffling of V-genes by using phage display technology and enables conversion of the murine antibody into completely human antibody with similar binding characteristics. Mouse VH and VL domains are used as epitope template to guide the selection of a human antibody-partner and are replaced sequentially or in parallel with human VH and VL domains, respectively. The resulting human antibodies, therefore, bind to epitopes that are at least overlapping with that of original mouse mAb. Thus, guided selection provides a powerful tool for humanization of the preexisting nonhuman antibodies of which biological activities are demonstrated.
Sequential Guided Selection for Humanization in Creative Biolabs
Creative Biolabs has developed a sequential guided selection procedure to derive completely human antibody. Mouse VL and VH domains are replaced sequentially with human VL and VH, respectively. The humanization procedure of a Fab format is as follows. 1) Clone the variable regions of a mouse antibody to a phagemid vector containing human constant domains; 2) Construct a human VL shuffled library by replacing the mouse VL with human VL repertoires; 3) Select binders after panning against a target antigen; 4) Construct a human VH shuffled library by replacing the mouse VH. 5) Select fully-human Fab clones after panning. Based on sequential guided selection, we have successfully selected a series of human antibodies with similar characteristics to the original non-human antibody.
Key Features and Advantages Including but Not Limited to
Creative Biolabs has more than ten years’ experience in antibody engineering for therapeutic and diagnostic development. We can offer our clients high-quality service of humanization of non-human antibodies by sequential guided selection at the most competitive price. Please feel free to contact us for more information.
Reference
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
