Creative Biolabs provides custom SFTI phage display library construction service with the best quality. Through our proprietary Hi-Affi™ phage display platform, the generated SFTI library can achieve high affinity and diversity.
Sunflower trypsin inhibitor (SFTI), a potent inhibitor of trypsin, is a small, ribosomally synthesized circular peptide of 14 amino acid isolated from the seeds of the sunflower Helianthus annuus. SFTI is the smallest known member of the Bowman-Birk family of serine protease inhibitors which interact with the enzymes they block via an exposed surface loop adopting the canonical protease inhibitor conformation. As a result, the noncovalent complex inactivates the protease. Another particular feature of the Bowman-Birk family proteins is the particularly well-defined bonding loop which is a short β-sheet region linked by disulfide bond.
SFTI scaffold can be a promising candidate for peptide-based drug design. As a small size peptide, SFTI comprises two short antiparallel β-strands connected at the reactive site end by an extended loop region, and by a hairpin turn at the opposite end. Its stability based on a cyclic backbone, a network of hydrogen bonds, a structure constrained by three proline residues and an intramolecular disulfide bridge for additional rigidity. SFTI is amenable to chemical synthesis, allowing for the creation of synthetic variants. The creation of linear variants of SFTI offers an option for studying the putative linear precursor peptides. In addition, SFTI may be considered as a possible scaffold structure for the future development of peptide-based radiopharmaceuticals.
Creative Biolabs has developed Hi-Affi™ phage display platform for scaffold protein library construction service. On the basis of general used exogenous gene expression method, which fusing the target genes to bacteriophage coat proteins then displaying on the phage surfaces to select specific binders, our scientists have innovatively combined trimer codon technology and NNK methods together to create this novel platform. Hi-Affi™ phage display platform can introduce more randomicity for phage library and is more suitable for sorting and isolating the high affinity protein or peptide targets. Commonly, we can obtain 100% precise mutant and over 1010 diversity for the constructed SFTI phage display library.
Creative Biolabs has extensive experience for offering library construction service with highest quality and most competitive price. We have generated more than 50 kinds of scaffold libraries for customers all over the world. Our scientists are confident in generating tailored scaffold protein library with the best quality and highest efficiency.
Fig. 1 High resolution structure of a potent, cyclic protease inhibitor from sunflower seeds. (PDB ID: 1SFI)