Anti-PTM Specific Antibody Introduction

Post-translational modifications (PTM) bring proteins more functions and activities. The development of anti-PTM antibodies is not an easy task, greatly hindering the PTM research. Creative Biolabs has extensive experience in antibody development. Based on our phage display antibody discovery platform, we can provide the highest quality anti-PTM antibody development services to advance your PTM research.

Anti-PTM Antibody Introduction

Protein PTM

PTM is a covalent modification process by adding a modifying group to one or more amino acid residues. PTM can alter the physical and chemical properties of proteins, which in turn affects the spatial conformation and activity state, subcellular localization, folding and stability, and protein-protein interactions. Common PTMs include phosphorylation, glycosylation, lipidation, acetylation, ubiquitination, and methylation modifications and allows proteins to have richer biological functions and roles.

Anti-PTM Antibody

Post-translationally modified proteins are often studied using antibodies. Although the initial confirmation of the modification can be performed at the beginning of the study using modification pan-antibodies. However, after confirmation of the modification site by mass spectrometry, custom site-specific antibodies are still needed for the confirmation of the modification and in-depth studies. However, the development of custom modified antibodies is not an easy task as they are difficult to develop due to the limited immunogen selection area and modification groups and are unique and original. The quality control of custom anti-PTM antibodies is also difficult to maintain.

Tips on Anti-PTM Antibody Development

Here are some tips to help generate satisfactory anti-PTM antibodies.

During the development of anti-PTM antibodies, if multiple modifications are detected on the target protein by mass spectrometry, we recommend selecting them according to the specific experimental requirements. In general, priority is given to sites with strong signals. If there are few sites to be selected, they can also be screened using modified pan-antibodies and point mutation experiments, and sites where the overall signal of the modification becomes significantly weaker after mutation may be more important. Second, preferentially select modification sites located within functional structural domains, such as catalytic structural domains, which are more likely to play an important regulatory function. Finally, preferring sites that are conserved across multiple species is also a reflection of their importance if a site has been preserved over a long evolutionary period.

When custom antibody hetero-bands are numerous, immunoprecipitation (IP) can be performed using the target protein first, followed by the incubation of custom modified antibodies.

When customizing antibodies, the choice between monoclonal and polyclonal antibodies is difficult. Polyclonal antibodies contain multiple antibodies, while monoclonal antibodies contain only one, and they both are essentially identical in use. The advantage of customizing polyclonal antibodies is the short development cycle and favorable price, while the advantage of customizing monoclonal antibodies is the high reproducibility between batches. Therefore, taking into account the place of use and development cost, we recommend developing monoclonal antibodies if the antibody is expected to be used in large quantities at a later stage, and customizing polyclonal antibodies is preferred if it is expected to be used only for the current subject.

More Details on Anti-membrane Protein Antibody

Phosphorylation-Specific Antibody Introduction	Acetylation-Specific Antibody Introduction
Glycosylation-Specific Antibody Introduction	Methylation-Specific Antibody Introduction
Ubiquitination-Specific Antibody Introduction	Sumoylation-Specific Antibody Introduction
Neddylation-Specific Antibody Introduction	Pupylation-Specific Antibody Introduction
ISGylation-Specific Antibody Introduction	S-Glutathionylation-Specific Antibody Introduction
S-Nitrosylation-Specific Antibody Introduction	Prenylation-Specific Antibody Introduction
Succinylation-Specific Antibody Introduction	Fatty Acylation-Specific Antibody Introduction
Tyrosine nitration-Specific Antibody Introduction	Polyglutamylation-Specific Antibody Introduction
Polyglycylation-Specific Antibody Introduction	Citrullination-Specific Antibody Introduction
Tyrosine Sulfation-Specific Antibody Introduction	ADP-Ribosylation-Specific Antibody Introduction
Adenylylation-Specific Antibody Introduction	Uridylylation-Specific Antibody Introduction
Formylation-Specific Antibody Introduction	Propionylation-Specific Antibody Introduction
Butyrylation-Specific Antibody Introduction	Malonylation-Specific Antibody Introduction
Crotonylation-Specific Antibody Introduction	Glutarylation-Specific Antibody Introduction
2-Hydroxyisobutyrylation-Specific Antibody Introduction	β-Hydroxybutyrylation-Specific Antibody Introduction
Carbamylation-Specific Antibody Introduction	Carboxylation-Specific Antibody Introduction
Diphthamide-Specific Antibody Introduction	Arginylation-Specific Antibody Introduction
Tyrosination-Specific Antibody Introduction	Phosphocholination-Specific Antibody Introduction

Creative Biolabs would like to share with you the key points and experiences in anti-PTM antibodies to facilitate the workflow of your project.

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.

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